A diketopyrrolopyrrole-based hybrid organic nanoprobe for ratiometric imaging of endogenous hypochlorite in live cells
A diketopyrrolopyrrole-based hybrid organic nanoprobe for ratiometric imaging of endogenous hypochlorite in live cells
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基于二酮吡咯并吡咯的混合有机纳米探针，用于活细胞内源性次氯酸盐的比率成像
DOI：
10.1016/j.snb.2019.127632
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Qu Junle
中科院分区：
文献类型：
--
作者：
Nie Kaixuan;Yuan Yufeng;Peng Xiao;Song Jun;Qu Junle
利用荧光共振能量转移（FRET）和聚集诱导发射（AIE）技术，将苯基二酮基吡咯并吡咯（PDPP 1）和两亲性四苯乙烯（TPE 1）在水溶液中共组装成有机胶束纳米探针，用于次氯酸盐的比率检测. PDPP 1是FRET纳米系统中的能量受体，具有很高的化学活性，以DPP核心的C双键为反应位点，对次氯酸盐具有快速、选择性和灵敏的响应。另一方面，TPE 1的巧妙引入不仅抑制了PDPP 1的ACQ效应，而且有助于保持纳米探针的稳定纳米结构。在次氯酸盐存在下，DPP核中的C双键C键被氧化，PDPP 1/TPE 1之间的FRET过程被破坏。这些PDPP 1/TPE 1纳米颗粒以比率方式对次氯酸盐快速响应，具有良好的灵敏度/选择性（LOD，32 nM），并且能够对活细胞中的外源性和内源性次氯酸盐进行成像。我们的研究提供了新的见解，以简单的建设比例纳米探针的生物分析与一些智能有机荧光团。
Using the fluorescence resonance energy transfer (FRET) and the aggregation-induced emission (AIE) strategies, we herein develop an organic micellar nanoprobe by the co-assembly of phenyl-diketopyrrolopyrrole (PDPP1) and amphiphilic tetraphenylethene (TPE1) in aqueous solution for ratiometic detection of hypochlorite. PDPP1, the energy acceptor in this FRET nanosystem, holds high chemical activity and responds rapidly, selectively and sensitively to hypochlorite with the Cdouble bondC double bond in DPP core as the reactive site. On the other side, the skillful introduction of TPE1 not only inhibits the ACQ effect of PDPP1, but also helps to maintain the stable nanostructure of nanoprobe. At the presence of hypochlorite, Cdouble bondC bonds in DPP core are oxidized and the FRET process between PDPP1/TPE1 is broken. These PDPP1/TPE1 nanoparticles respond rapidly to hypochlorite with good sensitivity/selectivity (the LOD, 32 nM) in the ratiometic way and are capable for imaging of exogenous and endogenous hypochlorite in live cells. Our research offers new insight into the simple construction of ratiometric nanoprobe for biological analyst with some smart organic fluorophores.