Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.
Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I.
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DOI：
10.1083/jcb.202011153
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发表时间：
2021-10-04
期刊：
影响因子：
--
通讯作者：
Jones KT
中科院分区：
文献类型：
--
作者：
Wu T;Lane SIR;Morgan SL;Tang F;Jones KT
Wu等人发现，在小鼠卵母细胞中，少量的卫星转录本在防止减数分裂i过程中中心点周围异染色质断裂中起保护作用。当这种断裂存在时，通过纺锤体组装检查点激活降低卵母细胞成熟率，并导致染色体分离缺陷。DNA着丝粒区域的重复序列构成着丝粒组装的结构基础。最近发现它们在有丝分裂中具有转录活性，其rna提供非编码功能。在这里，我们探讨的作用，在小鼠卵母细胞中，转录本产生的小卫星重复。少量卫星转录本的耗尽通过激活纺锤体组装检查点延迟了减数分裂I的进程。被阻滞的卵母细胞染色体聚集不良，着丝粒经常被微管分裂。因此，我们已经证明，与有丝分裂相比，着丝粒RNA在雌性减数分裂I中起着特殊的作用，并且是维持着丝粒结构完整性所必需的。这可能是在雌性减数分裂中观察到的高非整倍性的原因。
Wu et al. find that in mouse oocytes, minor satellite transcripts play a protective role in preventing pericentromeric heterochromatin from breaking during meiosis I. Such breaks, when present, reduce rates of oocyte maturation through spindle assembly checkpoint activation and lead to chromosomal segregation defects. The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes, and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared with mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis.
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α-卫星RNA转录本被Centromere-Nucleolus关联抑制。
影响因子：
7.7
作者：
Bury L;Moodie B;Ly J;McKay LS;Miga KH;Cheeseman IM
通讯作者：
Cheeseman IM
影响因子：
3.6
作者：
Balboula, Ahmed Z.;Blengini, Cecilia S.;Schindler, Karen
通讯作者：
Schindler, Karen
影响因子：
4.6
作者：
Lane, Simon I. R.;Yun, Yan;Jones, Keith T.
通讯作者：
Jones, Keith T.
影响因子：
16.6
作者：
Barra V;Fachinetti D
通讯作者：
Fachinetti D
DOI：
10.1242/dev.153965
发表时间：
2017-10-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Lane SIR;Morgan SL;Wu T;Collins JK;Merriman JA;ElInati E;Turner JM;Jones KT
通讯作者：
Jones KT