Analysis and Identification of Acid-Base Indicator Dyes by Thin-Layer Chromatography.
Analysis and Identification of Acid-Base Indicator Dyes by Thin-Layer Chromatography.
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薄层色谱法分析和鉴定酸碱指示染料。
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发表时间：
2007
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通讯作者：
D. D. Clark
中科院分区：
文献类型：
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作者：
D. D. Clark
薄层色谱法（TLC）是一种简单而有用的分离混合物中化合物的技术。有机化学家通常使用TLC来跟踪反应的进展，定性分析化合物或混合物，确定最佳色谱条件，并识别混合物中的组分。此外，薄层色谱法作为一种教育工具，在许多有机化学实验室课程中介绍色谱原理，具有既定的重要作用。因此，有机化学实验的发展，作为教育工具，有效地说明色谱原理是必要的。本文介绍了一种用于分析和鉴别常见酸碱指示染料的薄层色谱实验。本实验通过对颜色鲜艳的酸碱指示染料的拆分，并根据Rf值（与前体的比值）对染料进行鉴定，来说明色谱法的基本原理和实用性。
Thin-layer chromatography (TLC) is a simple and useful technique for the separation of compounds in a mixture. Organic chemists commonly employ TLC to follow the progression of a reaction, to qualitatively analyze a compound or mixture, to determine optimal chromatographic conditions, and to identify the components in a mixture. Additionally, TLC has an established and important role as an educational tool for the introduction of chromatographic principles in many organic chemistry laboratory curricula. Therefore, the development of organic chemistry laboratory experiments, as educational tools, that effectively illustrate chromatographic principles is essential. Presented herein is a TLC experiment that has been developed for the analysis and identification of common acid–base indicator dyes. This experiment serves to illustrate the fundamentals and utility of chromatography through the resolution of brightly colored acid–base indicator dyes and identification of the dyes on the basis of Rf (ratio to fro...