Possible role of immune surveillance at the initial phase of metastasis produced by B16BL6 melanoma cells
Possible role of immune surveillance at the initial phase of metastasis produced by B16BL6 melanoma cells
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DOI：
10.1016/s0014-5793(00)01144-3
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发表时间：
2000-02-11
期刊：
影响因子：
3.5
通讯作者：
Oku, N
中科院分区：
文献类型：
--
作者：
Kikkawa, H;Imafuku, H;Oku, N
检查了肺转移性B16 BL 6细胞的实时运输、转移潜力和注射的细胞数量之间的关系，因为注射的肿瘤细胞数量越少，可以观察到的免疫防御越明显。当通过尾静脉将1 × 10（6）或1 × 10（5）B16 BL 6细胞注射到小鼠体内时，两种细胞数量以相似的速率在肺中积累：两种剂量之间积累的细胞数量相差约10倍。从肺中消除不依赖于细胞数量，而是依赖于累积细胞的比例。然而，注射1 × 10（4）个细胞导致的肺蓄积小于注射1 × 10（5）个细胞获得的肺蓄积的十分之一。当注射1 X 10（5）或1 X 10（6）个B16 BL 6细胞时观察到转移，但注射1 X 10（4）个细胞后观察不到转移。为了阐明免疫防御系统在转移初期的作用，我们用1 × 10（4）个肿瘤细胞攻击巨噬细胞耗竭的小鼠。在肿瘤细胞攻击之前用2-氯腺苷治疗小鼠不仅取消了对转移的抑制，而且取消了对肺蓄积的抑制。此外，在肿瘤细胞攻击之后施用2-氯腺苷对转移潜力几乎没有影响。这些结果表明，免疫监视的作用是明显的，在低剂量的攻击肿瘤细胞的功能强，在转移过程的初始阶段，但不是在晚期阶段，巨噬细胞在抑制转移中发挥重要作用。(C)2000年欧洲生物化学学会联合会。
The relationship among the real-time trafficking of lung metastatic B16BL6 cells, metastatic potential, and the injected number of the cells was examined, since the smaller the number of tumor cells injected, the more clearly the immune defense may be observed. When 1 x 10(6) or 1 X 10(5) B16BL6 cells were injected into mice via the tail vein, both numbers of cells accumulated in the lung at a similar rate: there nas an approximately 10-fold difference in the number of accumulated cells between the two doses. Elimination from the lung was not dependent on the cell number but on the proportion of accumulated cells. However, the injection of 1 X 10(4) cells resulted in lung accumulation less than one-tenth of that obtained with 1 X 10(5) cell injection. Metastasis was observed when 1 X 10(5) or 1 X 10(6) B16BL6 cells were injected, but not after injection of 1 X 10(4) cells. To clarify the roles of the immune defense system at the initial phase of metastasis, we challenged macrophage-depleted mice with 1 X 10(4) tumor cells. Treatment of mice with 2-chloroadenosine prior to the tumor cell challenge cancelled the suppression of not only metastasis but also the lung accumulation, Furthermore, the administration of 2-chloroadenosine following the tumor cell challenge had little effect on the metastatic potential. These results suggest that the immune surveillance whose action was obvious at the low dose of challenged tumor cells functions strongly at the initial phase but not at the advanced stages of the metastatic process, and that macrophages play an important role in the suppression of metastasis. (C) 2000 Federation of European Biochemical Societies.