Attenuation of oscillatory potentials in nob2 mice.
Attenuation of oscillatory potentials in nob2 mice.
复制标题
nob2 小鼠振荡电位的衰减。
DOI：
10.1007/s10633-007-9058-9
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Peachey,NealS
中科院分区：
文献类型：
--
作者：
Yu,Minzhong;Peachey,NealS
PurposeTo examine changes in inner retinal function ofnob2mice, expressing a null mutation inCacna1fencoding the CaV1.4 subunit of voltage-dependent calcium channels.CACNA1Fmutations underlie one form of incomplete X-linked congenital stationary night blindness (CSNB2). In addition to a loss of dark-adapted (rod-driven) visual sensitivity, electroretinogram (ERG) b-waves and oscillatory potentials (OPs) are decreased in CSNB2 patients.MethodsERGs were recorded under dark-and light-adapted conditions from the corneal surface ofnob2mice, WT littermates andnob4mice. ERG frequency spectra were calculated by fast Fourier transform (FFT). A FFT-based high-pass filter was used to derive OP waveforms.ResultsUnder dark-adapted conditions, the dominant frequency of the OPs varied between 90 to 120 Hz in WT mice. In WT mice, OP frequency first increased with flash intensity and then decreased at the highest flash levels while overall OP amplitude increased monotonically with increasing flash intensity. In response to low stimulus flashes, reliable OPs were not obtained fromnob2mice. OPs were only seen at stimulus intensities at or above −1.8 log cd s/m2, where they occurred at a lower frequency range (70–90 Hz) than for WT mice. When flash stimuli were superimposed against a steady rod-desensitizing adapting field, the amplitude and frequency of WT OPs increased with flash intensity above 0.4 log cd s/m2. In comparison to WT results, cone-mediated OPs obtained fromnob2mice were smaller in amplitude, of lower frequency and had delayed implicit times. We compared the extent to which OPs and the b-wave were reduced innob2mice, by normalizing to the results obtained from WT mice. In comparison to the b-wave, the OPs were relatively spared, under both dark- and light-adapted conditions.ConclusionsInnob2mice, rod- and cone-driven OPs are reduced in amplitude and occur at a lower frequency range. Since CaV1.4 is expressed in both the inner and outer plexiform layers, these changes are likely to reflect reduced transmission from photoreceptors to bipolar cells as well as alterations in inner retinal function. That the OPs were better preserved than b-waves suggests that inner retinal pathways may be reorganized in response to the decreased bipolar cell response innob2mice.
