Production of -linolenic acid in Yarrowia lipolytica using low-temperature fermentation
Production of -linolenic acid in Yarrowia lipolytica using low-temperature fermentation
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DOI：
10.1007/s00253-018-9349-y
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发表时间：
2018-10-01
影响因子：
5
通讯作者：
Alper, Hal S.
中科院分区：
文献类型：
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作者：
Cordova, Lauren T.;Alper, Hal S.
阿尔法-亚麻酸(ALA)是一种必需的脂肪，据报道对健康有好处。然而，这种分子天然存在于亚麻籽和油菜籽等植物中，目前限制了产量。在这里，我们展示了使用产油酵母解脂亚罗维菌可持续生产丙氨酸的潜力。通过使用最近发现的12-15脱饱和酶(RK12-15)，我们能够在解脂耶尔森菌中生产。当与先前设计的具有高前体利用率的高脂高产菌株相结合时，ALA的生产得到了进一步的改善。最后，该菌株在较低温度下的培养显著提高了ALA的含量，在20℃下发酵的细胞积累了近30%的ALA。这种低温发酵使ALA效价比标准生长条件提高了3.2倍。放大到补料分批生物反应器中，生产的丙氨酸为1.4g/L，是酵母宿主中这种-3脂肪酸的最高公布效价。
alpha-Linolenic acid (ALA) is an essential -3 fatty with reported health benefits. However, this molecule is naturally found in plants such as flaxseed and canola which currently limits production. Here, we demonstrate the potential to sustainably produce ALA using the oleaginous yeast Yarrowia lipolytica. Through the use of a recently identified 12-15 desaturase (Rk 12-15), we were able to enable production in Y. lipolytica. When combined with a previously engineered lipid-overproducing strain with high precursor availability, further improvements of ALA production were achieved. Finally, the cultivation of this strain at lower temperatures significantly increased ALA content, with cells fermented at 20 degrees C accumulating nearly 30% ALA of the total lipids in this cell. This low-temperature fermentation represents improved ALA titer up to 3.2-fold compared to standard growth conditions. Scale-up into a fed-batch bioreactor produced ALA at 1.4g/L, representing the highest published titer of this -3 fatty acid in a yeast host.y