Boar variability affects sperm metabolism activity in liquid stored semen at 5°C
Boar variability affects sperm metabolism activity in liquid stored semen at 5°C
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DOI：
10.2478/v10181-011-0003-1
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发表时间：
2011-01-01
影响因子：
0.8
通讯作者：
Strzezek, J.
中科院分区：
文献类型：
--
作者：
Dziekonska, A.;Strzezek, J.
使用生物发光法测定 ATP 含量、荧光测定（JC 荧光染料）测定线粒体活性和耗氧量，测量公猪精子的代谢活性（液体在 5 摄氏度下储存三天）。同时分析精子活力和质膜完整性（PMI）。除了精液储存时间具有统计显着性影响（P < 0.001）之外，还显示了精液的个体来源对于精子代谢效率分析参数的重要性。这种现象表现为精液的个体来源与精子活力、膜完整性和代谢活动与精液保存时间的相互作用。记录结果表明，个体因素可能对公猪精子液体储存的技术用途产生重大影响。对储存在 5 摄氏度的公猪精液进行的质量分析可用于预选产生对冷休克耐受性增强的精子的公猪。
Metabolic activity of boar spermatozoa, liquid stored for three days at 5 degrees C, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5 degrees C may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.