Adoptive transfer of unresponsiveness to allogeneic skin grafts with hepatic gamma delta + T cells.
Adoptive transfer of unresponsiveness to allogeneic skin grafts with hepatic gamma delta + T cells.
复制标题
将无反应性过继转移至带有肝 γ δ T 细胞的同种异体皮肤移植物。
作者：
R. Gorczynski
经门静脉注射经辐照的多种次要不相容B10.BR淋巴细胞的C3 H/HEJ小鼠显示后续B10.BR皮肤移植物的延迟排斥。通过侧尾静脉注射B10. BR肝单个核细胞或用B10次要组织相容性抗原在体内脉冲的H-2k细胞产生类似的延迟排斥。体内C3 H抗B10.BR免疫的抑制（通过延迟移植物排斥反应评估）和体外（通过B10评估。BR诱导的淋巴因子产生）可以通过辐射抗性，塑料贴壁F4/80+ 33 D1-CD 4-CD 8-α β TcR-γ δ TcR-单核肝细胞，来自（C3 H/HEJ x C3H.SW）F1小鼠提前36小时注射100 x 10（6）个辐照脾细胞。到注射后10天，转移延迟排斥的细胞是放射敏感的、可塑性非粘附的F4/80- 33 D1-CD 4-CD 8-α β Tc+-γ δ TcR+细胞。通过门静脉向接受B10.BR细胞预处理的小鼠体内注射白细胞介素-2（IL-2），或将免疫抗B10.BR淋巴细胞过继转移到此类小鼠中，可消除随后皮肤移植时的延迟排斥反应。在所有情况下，延迟排斥反应或调节淋巴因子的产生与抑制IL-2的产生和优先保留体外刺激细胞产生的IL-4有关。
C3H/HEJ mice injected with irradiated multiple minor incompatible B10.BR lymphoid cells via the portal vein showed delayed rejection of subsequent B10.BR skin grafts. Similar delayed rejection was produced by lateral tail vein injection of B10.BR hepatic mononuclear cells or H-2k cells pulsed in vivo with B10 minor histocompatibility antigens. Inhibition of C3H anti-B10.BR immunity in vivo (assessed by delayed graft rejection) and in vitro (assessed by B10.BR-induced lymphokine production) can be transferred by radioresistant, plastic-adherent F4/80+33D1-CD4-CD8-alpha beta TcR-gamma delta TcR- mononuclear hepatic cells from (C3H/HEJ x C3H.SW)F1 mice injected 36 hr earlier with 100 x 10(6) irradiated spleen cells. By 10 days post-injection, cells transferring delayed rejection are radiosensitive, plastic non-adherent, F4/80-33D1-CD4-CD8- alpha beta Tc+- gamma delta TcR+ cells. Injection of interleukin-2 (IL-2) in vivo into mice receiving pretreatment with B10.BR cells via the portal vein, or adoptive transfer into such mice of immune anti-B10.BR lymphoid cells, abolished delayed rejection on subsequent skin grafting. Delayed rejection or modulation of lymphokine production was associated in all cases with suppression of IL-2 production and preferential retention of IL-4 production from cells stimulated in vitro.