Spatial differences in gene expression in the bovine oviduct
Spatial differences in gene expression in the bovine oviduct
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DOI：
10.1530/rep-16-0074
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发表时间：
2016-07-01
期刊：
影响因子：
3.8
通讯作者：
Rizos, Dimitrios
中科院分区：
文献类型：
--
作者：
Maillo, Veronica;de Frutos, Celia;Rizos, Dimitrios
本研究的目的是比较怀孕小母牛输卵管峡部的转录组与循环小母牛的转录组，并研究怀孕小母牛输卵管峡部和壶腹部转录组的空间差异。同步杂交小母牛后，将处于发情期（=第 0 天）的小母牛随机分配到循环组（非繁殖组，n = 6）或怀孕组（人工授精组，n = 11）。在第3天屠宰它们，分离每只动物的两个输卵管并切成两半以分离壶腹部和峡部。冲洗每个部分以确认卵母细胞/胚胎的存在，然后纵向打开并刮擦以获得上皮细胞，将其速冻。卵母细胞和胚胎位于黄体同侧的输卵管峡部。输卵管细胞的微阵列分析表明，无论妊娠状态如何，靠近黄体不会影响峡部的转录组。然而，妊娠动物黄体同侧输卵管壶腹部和峡部之间有 2287 个基因存在差异表达（P < 0.01）。基因本体论显示，峡部的主要生物过程是氮、脂质、核苷酸、类固醇和胆固醇的合成，以及囊泡介导的运输、细胞周期、细胞凋亡、胞吞作用和胞吐作用，而壶腹部的主要生物过程是细胞运动、运动和迁移、DNA修复、钙离子稳态、碳水化合物生物合成以及纤毛运动和搏动频率的调节。总之，在发情后第3天，怀孕动物峡部和壶腹之间的基因表达存在较大差异。
The aim of this study was to compare the transcriptome of the oviductal isthmus of pregnant heifers with that of cyclic heifers as well as to investigate spatial differences between the transcriptome of the isthmus and ampulla of the oviduct in pregnant heifers. After synchronizing crossbred beef heifers, those in standing oestrus (=Day 0) were randomly assigned to cyclic (non-bred, n = 6) or pregnant (artificially inseminated, n = 11) groups. They were slaughtered on Day 3 and both oviducts from each animal were isolated and cut in half to separate ampulla and isthmus. Each portion was flushed to confirm the presence of an oocyte/embryo and was then opened longitudinally and scraped to obtain epithelial cells which were snap-frozen. Oocytes and embryos were located in the isthmus of the oviduct ipsilateral to the corpus luteum. Microarray analysis of oviductal cells revealed that proximity to the corpus luteum did not affect the transcriptome of the isthmus, irrespective of pregnancy status. However, 2287 genes were differentially expressed (P < 0.01) between the ampulla and isthmus of the oviduct ipsilateral to the corpus luteum in pregnant animals. Gene ontology revealed that the main biological processes overrepresented in the isthmus were synthesis of nitrogen, lipids, nucleotides, steroids and cholesterol as well as vesicle-mediated transport, cell cycle, apoptosis, endocytosis and exocytosis, whereas cell motion, motility and migration, DNA repair, calcium ion homeostasis, carbohydrate biosynthesis, and regulation of cilium movement and beat frequency were overrepresented in the ampulla. In conclusion, large differences in gene expression were observed between the isthmus and ampulla of pregnant animals at Day 3 after oestrus.