Differential expression of three Chlamydia trachomatis hsp60-encoding genes in active vs. persistent infections
Differential expression of three Chlamydia trachomatis hsp60-encoding genes in active vs. persistent infections
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DOI：
10.1016/j.micpath.2003.08.005
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发表时间：
2004-01-01
影响因子：
3.8
通讯作者：
Hudson, AP
中科院分区：
文献类型：
--
作者：
Gérard, HC;Whittum-Hudson, JA;Hudson, AP
使用实时 RT-PCR 来评估三种沙眼衣原体 hsp60 编码基因（Ct110、0604、Ct755）在活动性与持续性感染的体外系统中以及衣原体诱导的关节炎患者的滑液样本中随时间的变化情况的表达。在主动感染沙眼衣原体（血清型 K）的 HEp-2 细胞中，Ct110 (groEL) 的 mRNA 在感染后 8 小时 (pi.i.) 时明显可见，并在感染后 48 小时内增加了 10 倍以上； Ct604 的 mRNA 遵循类似的模式。 0755 的转录本在感染后 8 小时非常丰富。并且始终比 Ct110 的结果高 2-3 倍。在培养物中持续感染的人单核细胞中，Ct110 和 0755 的表达在注射后 1 至 7 天内较低，而来自 0604 的 mRNA 在注射后 1 天内丰富。并且随着生物体过渡到持久状态，从 1 d 到 3 d p.i. 增加了 3 倍以上。这些 mRNA 水平在注射后 7 天内仍然很高。对衣原体相关关节炎患者滑膜组织的 RNA/cDNA 进行实时分析，结果显示 0604 mRNA 水平较高，这与体外单核细胞持久性系统的结果一致。这些数据表明，每个衣原体 hsp60 编码基因都是独立表达的，并且这三个基因在活动性感染和持续性感染中的表达存在差异。结果进一步表明0604基因产物可能在衣原体持续存在期间发挥重要作用。 (C) 2004 Elsevier Ltd. 保留所有权利。
Real time RT-PCR was used to assess expression of the three Chlamydia trachomatis hsp60-encoding genes (Ct110, 0604, Ct755) over time in in vitro systems of active vs. persistent infection, and in synovial samples from patients with Chlamydia-induced arthritis. In HEp-2 cells actively infected with C. trachomatis (serovar K), mRNA from Ct110 (groEL) was apparent by 8 h post-infection (p.i.) and increased more than 10-fold through 48 h p.i.; mRNA from Ct604 followed a similar pattern. Transcripts from 0755 were abundant at 8 h p.i. and remained 2-3-fold higher than those from Ct110 at all times. In persistently infected human monocytes in culture, expression of Ct110 and 0755 was low from 1 to 7 d p.i., whil e mRNA from 0604 was abundant at I d p.i. and increased more than 3-fold from I to 3 d p.i., as the organism transited to the persistent state. Those mRNA levels remained high through 7 d p.i. Real time analyses of RNA/cDNA from synovial tissue of patients with Chlamydia-associated arthritis showed high 0604 mRNA levels, consistent with results from the in vitro monocyte system of persistence. These data demonstrate that each chlamydial hsp60-encoding gene is expressed independently, and that the three genes are expressed differentially in active vs. persistent infection. The results further suggest that the 0604 gene product may function importantly during chlamydial persistence. (C) 2004 Elsevier Ltd. All rights reserved.