Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages
Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages
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DOI：
10.1016/j.jmii.2013.03.011
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发表时间：
2014-10-01
影响因子：
7.4
通讯作者：
Chen, Ssu Ching
中科院分区：
文献类型：
--
作者：
Hseu, You-Cheng;Sung, Jia-Chuen;Chen, Ssu Ching
背景/目的：在这项研究中，我们探讨了类鼻疽伯克霍尔德氏菌细胞感染的小鼠巨噬细胞是否会诱导细胞凋亡相关基因和蛋白的产生。方法：用B.分别在0.5小时、1小时、2小时、4小时和6小时对假鼻疽细胞进行细胞培养，随后进行实时聚合酶链反应（PCR）阵列分析。Western blot检测凋亡相关蛋白caspase-3、caspase-8、caspase-9、Bax和Bcl-2的表达。同时用PCR芯片检测了其他相关基因的表达水平。实验结果表明，肿瘤坏死因子配体（例如，结论：B. tnfsf 10和tnfrs 10 b）和Fas的表达水平升高，而Birc 5、Hells和Bnip 3等抗凋亡基因的表达水平降低。假鼻疽Copyright（C）2013，Taiwan Society of Microbiology.出版社：Elsevier Taiwan LLC All rights reserved.
Background/Purpose: In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells.Methods: Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot.Results: After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased.Conclusion: Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei. Copyright (C) 2013, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.