Coherent Movement of Cell Layers during Wound Healing by Image Correlation Spectroscopy
Coherent Movement of Cell Layers during Wound Healing by Image Correlation Spectroscopy
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DOI：
10.1016/j.bpj.2009.06.052
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发表时间：
2009-10-07
影响因子：
3.4
通讯作者：
Gratton, Enrico
中科院分区：
文献类型：
--
作者：
Tanner, Kandice;Ferris, Donald R.;Gratton, Enrico
我们已经确定了蝾螈皮肤外植体模型中从损伤点到再上皮化的复杂事件顺序，并表明细胞层在损伤后由细胞肿胀驱动而一致移动。我们使用相关光谱量化了三维细胞迁移，并解决了复杂的动力学问题，例如位错点的形成和协调的细胞运动。我们量化了愈合过程中细胞的速度和肿胀等相对行为作为细胞层的函数。我们认为，细胞体积的增加（类似于基底层的 37%）是损伤后细胞迁移开始的驱动力，其中增大的细胞产生一个错位点，预示并决定了细胞迁移的初始方向。总体而言，细胞遵循伤口闭合后维持的一致涡流运动。我们的结果表明细胞体积改变了损伤后细胞的迁移。
We have determined the complex sequence of events from the point of injury until reepithelialization in axolotl skin explant model and shown that cell layers move coherently driven by cell swelling after injury. We quantified three-dimensional cell migration using correlation spectroscopy and resolved complex dynamics such as the formation of dislocation points and concerted cell motion. We quantified relative behavior such as velocities and swelling of cells as a function of cell layer during healing. We propose that increased cell volume (similar to 37% at the basal layer) is the driving impetus for the start of cell migration after injury where the enlarged cells produce a point of dislocation that foreshadows and dictates the initial direction of the migrating cells. Globally, the cells follow a concerted vortex motion that is maintained after wound closure. Our results suggest that cell volume changes the migration of the cells after injury.