Dietary choline supplementation in pregnant rats increases hippocampal phospholipase D activity of the offspring
Dietary choline supplementation in pregnant rats increases hippocampal phospholipase D activity of the offspring
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DOI：
10.1096/fasebj.10.14.9002559
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发表时间：
1996-12-01
期刊：
影响因子：
4.8
通讯作者：
Blusztajn, JK
中科院分区：
文献类型：
--
作者：
Holler, T;Cermak, JM;Blusztajn, JK
大鼠妊娠期补充胆碱可长期改善后代的视觉空间记忆，其生化机制可能与胆碱作为磷脂酰胆碱（PC）前体的功能有关，PC是受体刺激酶磷脂酶D （PLD）的底物，PLD的激活启动了细胞内两种信使磷脂酸和1,2-sn-二酰基甘油的顺序形成。我们假设产前胆碱状态可能导致海马中PLD催化的PC水解的长期调节，海马是一个涉及视觉空间记忆功能的大脑区域，通过测量PLD在l-丙醇存在下催化的[3H]磷脂酰丙醇的形成，在预先标记了[H-3]甘油或[H-3]油酸的海马切片中测定了PLD的活性。或choline-free饮食从天11到17岁的怀孕、磷脂类的放射性标记是治疗的影响,产前补充胆碱显著增加基底骑士活动在[H-3] glycerol-labeled片[46%的控制产后7天(P)和36%第21页),和[H-3] oleate-labeled片(91% P7),以及glutamate-stimulated骑士活动在[H-3] oleate-labeled片(60% P ?),产前胆碱不足未能改变骑士活动,胆碱的作用显然需要完整的细胞，因为使用荧光NBD-PC作为底物对海马均质液中PLD活性的体外检测显示各组之间没有差异。结果表明，在出生后发育过程中，产前补充胆碱可以上调海马中基础和受体刺激的PLD活性。
Supplementation with choline during pregnancy in rats causes a long-lasting improvement of visuospatial memory of the offspring, The biochemical mechanism of this effect may be related to the function of choline as a precursor of phosphatidylcholine (PC), the substrate of a receptor-stimulated enzyme, phospholipase D (PLD), PLD activation initiates the sequential formation of two intracellular messengers, phosphatidic acid and 1,2-sn-diacylglycerol, We hypothesized that prenatal choline status may cause long-term modulation of PLD-catalyzed PC hydrolysis in the hippocampus, a brain region implicated in visuospatial memory functions, PLD activity was determined in hippocampal slices prelabeled with [H-3]glycerol or [H-3]oleic acid by measuring the PLD-catalyzed formation of [3H]phosphatidylpropanol in the presence of l-propanol, Slices were obtained from male pups born to mothers consuming a control diet, a choline-supplemented diet, or a choline-free diet from days 11 to 17 of pregnancy, The radiolabeling of phospholipid classes was unaffected by the treatments, Prenatal choline supplementation significantly increased basal PLD activity in [H-3]glycerol-labeled slices [by 46% of controls on postnatal day (P) 7 and by 36% on P21], and [H-3] oleate-labeled slices (by 91% on P7), as well as glutamate-stimulated PLD activity in [H-3]oleate-labeled slices (by 60% on P?), Prenatal choline deficiency failed to alter PLD activity, The actions of choline apparently required intact cells because in vitro assays of PLD activity in hippocampal homogenates, using fluorescent NBD-PC as substrate, revealed no differences between groups, The results show that prenatal choline supplementation up-regulates basal and receptor-stimulated PLD activity in the hippocampus during postnatal development.