Synthesis and release of thymidine by macrophages.
Synthesis and release of thymidine by macrophages.
复制标题
巨噬细胞合成和释放胸苷。
作者：
M. Stadecker;J. Calderón;M. Karnovsky;E. Unanue
对巨噬细胞上清中发现的DNA合成和细胞增殖抑制剂进行进一步分析。得出以下结论：a.从生物化学角度来看，从三种不同薄层体系的迁移和凝胶过滤来看，抑制剂似乎是胸苷。b.该抑制剂被证明是由巨噬细胞单层用放射性标记的胸苷前体（如14c -甲酸酯）脉冲重新合成的。c.组织培养液中存在的抑制剂的量被证明足以阻止EL-4白血病细胞系的生长，这种方式可以通过添加2'-脱氧胞苷来阻止。EL-4细胞系对胸苷阻断的敏感性明显高于许多不同的细胞。
An inhibitor of DNA synthesis and cell proliferation found in macrophage supernatants was subjected to further analysis. The following conclusions were drawn: a. Biochemically, the inhibitor appeared to be thymidine, judging from the migration in three different thin-layer systems and on gel filtration. b. The inhibitor was shown to be synthesized de novo by macrophage monolayers pulsed with a radiolabeled precursor of thymidine such as 14C-formate. c. The amount of inhibitor present in tissue culture fluids proved to be sufficient to block the growth of the EL-4 leukemia cell line in a manner that could be prevented with the addition of 2'-deoxycytidine. The EL-4 line was considerably more sensitive to thymidine blockade than a number of different cells tested.