A morpholino hydrazone-based lysosome-targeting fluorescent probe with fast response and high sensitivity for imaging peroxynitrite in living cells
A morpholino hydrazone-based lysosome-targeting fluorescent probe with fast response and high sensitivity for imaging peroxynitrite in living cells
复制标题
基于吗啉代腙的溶酶体靶向荧光探针，具有快速响应和高灵敏度，用于活细胞中的过氧亚硝酸盐成像
DOI：
10.1016/j.saa.2021.120100
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Youming Shen
中科院分区：
文献类型：
--
作者：
Biao Gu;Cuiyan Wu;Chunxiang Zhang;Shihui He;Siping Tang;Haitao Li;Youming Shen
Peroxynitrite (ONOO−) plays important roles in many pathophysiological processes and its subcellular detection draws increasing attention. In this study, we designed and prepared a novel lysosome-targetable fluorescent probe (E)-2-(benzo[d]thiazol-2- yl)-4-methyl-6-((morpholinoimino)methyl)phenol (BMP) for selective detection of ONOO−in living systems by incorporating a reactive morpholino hydrazone as new ONOO−response site into a benzothiazole derivative as fluorophore. After reaction with ONOO−, an obvious fluorescence increase (83-fold) was observed accompanied with distinct dual colorimetric and fluorescence changes. ProbeBMPdisplayed the merits of fast response (<3 s), ultrasensitivity (LOD = 6 nM) and high selectivity towards ONOO−over other physiological species including ROS/RNS. Most importantly, the probe was capable of imaging ONOO−in lysosomes of living cells with good cell permeation and negligible cytotoxicity. Therefore, this research provides an effective tool to study the functions of ONOO−in lysosomes.
