GENETIC AND ANTIGENIC ANALYSIS OF AN EQUINE INFLUENZA H-3 ISOLATE FROM THE 1989 EPIDEMIC
GENETIC AND ANTIGENIC ANALYSIS OF AN EQUINE INFLUENZA H-3 ISOLATE FROM THE 1989 EPIDEMIC
复制标题
DOI：
10.1007/bf01318994
复制
发表时间：
1993-01-01
影响因子：
2.7
通讯作者：
DANIELS, RS
中科院分区：
文献类型：
--
作者：
BINNS, MM;DALY, JM;DANIELS, RS
利用逆转录聚合酶链式反应技术克隆了马流感H3N8株Suffolk/89的血凝素(HA)基因。HA基因的核苷酸序列是从该基因的两个独立克隆副本中确定的，并被发现与最近的美国分离株关系最密切，支持大多数马H3N8分离株作为单一谱系进化的观点。当检查预测的Suffolk/89 HA的氨基酸序列时，与当前疫苗(Miami/63和Fontainebleau/79)中使用的分离株的序列相比，至少四个主要抗原位点A、B、C和D发生了变化。令人惊讶的是，当萨福克/分离株与一组6个鼠单抗进行血凝抑制(HI)试验时，萨福克/分离株与枫丹白露/79分离株之间没有观察到差异，这表明这组单抗可能识别有限的主要抗原点亚集。三个抗HA马的杂交瘤单克隆能够区分Suffolk/89和Fontainebleau/79病毒，表明马确实认识到这些分离株在抗原性上是不同的。这项工作的结果表明，当前马流感疫苗中使用的分离株可能需要更新。
The haemagglutinin (HA) gene from the equine influenza H3N8 isolate Suffolk/89 has been cloned by reverse transcription and polymerase chain reaction amplification. The nucleotide sequence of the HA gene was determined from two independently cloned copies of the gene and was found to be most closely related to recent American isolates supporting the idea that most isolates of equine H3N8 are evolving as a single lineage. When the predicted amino acid sequence of the Suffolk/89 HA was examined, changes had taken place in at least four of the major antigenic sites, A, B, C, and D when compared to the sequences of the isolates used in the current vaccines (Miami/63 and Fontainebleau/79). Surprisingly, when the Suffolk/89 isolate was tested in haemagglutination inhibition (HI) assays with a panel of six mouse monoclonal antibodies, no differences were observed between the Suffolk/89 and the Fontainebleau/79 isolates, suggesting that this panel of monoclonal antibodies may recognise a limited subset of the major antigenic sites. Three anti-HA horse heterohybridoma monoclonals were able to distinguish between the Suffolk/89 and Fontainebleau/79 viruses, demonstrating that the horse does recognise these isolates as being antigenically different. The results of the work suggest that the isolates used in current equine influenza vaccines may need updating.