Monitoring structural changes of proteins in an ion trap over ∼10-200 ms:: Unfolding transitions in cytochrome c ions
Monitoring structural changes of proteins in an ion trap over ∼10-200 ms:: Unfolding transitions in cytochrome c ions
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DOI：
10.1021/ac010744a
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发表时间：
2001-12-15
影响因子：
7.4
通讯作者：
Clemmer, DE
中科院分区：
文献类型：
--
作者：
Badman, ER;Hoaglund-Hyzer, CS;Clemmer, DE
本文介绍了一种研究气相蛋白质离子构象变化随时间变化的新技术。在这种方法中，一个短脉冲的电喷雾蛋白质离子被引入到离子陷阱中并存储。在规定的时间段后，离子的分布从陷阱被喷射到离子迁移率/飞行时间质谱仪中。质谱仪中迁移率和飞行时间的联合测量提供了关于不同构象类型和电荷状态分布的丰度的信息。通过改变陷阱中的存储时间，可以监测在延长的时间段(类似于10-200ms)内发生的离子构象的变化。该方法是通过检测+7到+10电荷态的细胞色素c离子构象的变化来演示的。
A new technique for studying the time dependence of conformational changes of gas-phase protein ions is described. In this approach, a short pulse of electrosprayed protein ions is introduced into an ion trap and stored. After a defined time period, the distribution of ions is ejected from the trap into an ion mobility/time-of-flight mass spectrometer. Combined measurements of mobilities and flight times in the mass spectrometer provide information about the abundances of different conformer types and charge-state distributions. By varying the storage time in the trap, it is possible to monitor changes in ion conformation that occur over extended time periods (similar to 10-200 ms). The method is demonstrated by examining changes in cytochrome c ion conformations for the +7 to +10 charge states.