Comparison of IgE values as determined by different solid phase radioimmunoassay methods
Comparison of IgE values as determined by different solid phase radioimmunoassay methods
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不同固相放射免疫分析方法测定IgE值的比较
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
N. Kjellman
中科院分区：
文献类型：
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作者：
S. Johansson;Asta Berglund;N. Kjellman
准确测量低IgE浓度在技术上是困难的。本文比较了放射免疫分析的直接夹心法和三种抑制法的结果。对于50U/ml以上的值，所有方法均具有良好的相关性。然而，在50U/ml以下，抑制方法往往会产生虚假的高值。对于很低的浓度，1-10U/ml的直接夹心试验(PRIST*)和抑制试验之间的相关性最好，该试验使用了一个校正因子来考虑血清的非特异性影响。
Accurate measurement of low IgE concentrations is technically difficult. In this paper results obtained by a direct sandwich and three inhibition methods of radioimmunoassays are compared. For values above 50 U/ml good correlation was obtained with all methods. Below 50 U/ml, however, the inhibition methods tended to yield falsely high values. For very low concentrations, 1–10 U/ml the best correlation was obtained between the direct sandwich test (PRIST*) and the inhibition test using a correction factor to allow for the non‐specific effect of serum.