INTERACTION OF TRANSFER-RNA WITH 23S RIBOSOMAL-RNA IN THE RIBOSOMAL A-SITES, P-SITES, AND E-SITES
INTERACTION OF TRANSFER-RNA WITH 23S RIBOSOMAL-RNA IN THE RIBOSOMAL A-SITES, P-SITES, AND E-SITES
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DOI：
10.1016/0092-8674(89)90128-1
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发表时间：
1989-05-19
期刊：
影响因子：
64.5
通讯作者：
NOLLER, HF
中科院分区：
文献类型：
--
作者：
MOAZED, D;NOLLER, HF
23 rRNA中的三组保守核苷酸通过tRNA分别与核糖体A、P和E位点结合而免受化学探针的影响。它们几乎完全位于结构域V中，主要位于肽基转移酶功能鉴定的环中或与其相邻。其中一些位点也受到抗生素如氯霉素的保护，这可以解释这些药物如何干扰蛋白质合成。当3“-末端A或CA或2”，3“-连接的酰基被去除时，某些tRNA依赖性保护被废除，为tRNA的保守CCA末端与23 S rRNA的相互作用提供了直接证据。当EF-Tu. cntdot. GTP.cntdot.当氨酰-tRNA三元复合物与核糖体结合时，在23 S rRNA中未检测到tRNA依赖性A位点保护，直到EF-Tu被释放。因此，EF-Tu在反密码子选择过程中阻止了进入的氨酰-tRNA的3“-末端与核糖体的肽基转移酶区域的相互作用，从而允许翻译校对。
Three sets of conserved nucleotides in 23 rRNA are protected from chemical probes by binding of tRNA to ribosomal A, P, and E sites, respectively. They are located almost exclusively in domain V, primarily in or adjacent to the loop identified with the peptidyl transferase function. Some of these sites are also protected by antibiotics such as chloramphenicol, which could explain how these drugs interfere with protein synthesis. Certain tRNA-dependent protections are abolished when the 3''-terminal A or CA or 2'',3''-linked acyl group is removed, providing direct evidence for the interaction of the conserved CCA terminus of tRNA with 23S rRNA. When the EF-Tu .cntdot. GTP .cntdot. aminoacyl-tRNA ternary complex is bound to the ribosome, no tRNA-dependent A site protections are detected in 23S rRNA until EF-Tu is released. Thus, EF-Tu prevents interaction of the 3''-terminus of the incoming aminoacyl-tRNA with the peptidyl transferase region of the ribosome during anticodon selection, thereby permitting translational proofreading.