Antitumor activity on human bladder cancer T-24 cells and composition analysis of the core ofCamellia osmanthafruit
Antitumor activity on human bladder cancer T-24 cells and composition analysis of the core ofCamellia osmanthafruit
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DOI：
10.1080/14786419.2018.1550763
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发表时间：
2020-09-16
影响因子：
2.2
通讯作者：
Pan, Ying-Ming
中科院分区：
文献类型：
--
作者：
Ouyang, Xi-Lin;Yang, Li;Pan, Ying-Ming
本研究对桂花果核的抗肿瘤活性进行了研究，并对桂花果核的化学成分进行了分析。用80%乙醇提取果核，得到粗提物（TF），用MCI树脂依次用不同浓度的乙醇分配，得到3个不同的部分（Frs 1 - 3）。细胞毒活性测定结果表明，TF、Fr-2和Fr-3对T-24细胞有较好的抑制作用，IC（50）值分别为6.7 ± 1.3、6.9 ± 0.9和6.7 ± 1.7 μ g/mL，Fr-3对T-24细胞有较强的抑制作用，主要是通过有效增加细胞内钙离子和活性氧的释放。此外，Fr-3还能抑制T-24细胞的迁移和侵袭。进一步用LC-Q-TOFMS对Fr-3进行了成分分析，表明其主要成分为鞣花酸及其衍生物。
In this study, antitumor activity and composition analysis of the core ofCamellia osmanthafruit were investigated. The cores were extracted by 80% ethanol to obtain the crude extract (TF), which was successively distributed by different concentrations of ethanol with MCI resin to obtain three different parts (Frs 1to3). Cytotoxicity activities showed thatTF,Fr-2, andFr-3exhibited good inhibition against T-24 with IC(50)values of 6.7 +/- 1.3, 6.9 +/- 0.9, and 6.7 +/- 1.7 mu g/mL, respectively.Fr-3has a strong inhibitory activity on T-24 cells, mainly due to effectively increasing the release of intracellular calcium ions and reactive oxygen species. In addition,Fr-3can inhibit T-24 cells migration and invasion. Further composition analysis onFr-3was detected by LC-Q-TOFMS implying the main components to be ellagic acid and its derivatives.