Association between the DQA MHC class II gene and Puumala virus infection in Myodes glareolus, the bank vole
Association between the DQA MHC class II gene and Puumala virus infection in Myodes glareolus, the bank vole
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DOI：
10.1016/j.meegid.2007.07.003
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发表时间：
2008-07-01
影响因子：
3.2
通讯作者：
Charbonnel, Nathalie
中科院分区：
文献类型：
--
作者：
Deter, Julie;Bryja, Josef;Charbonnel, Nathalie
普玛拉病毒(Puumala Virus，PUUV)是一种汉坦病毒，由库田鼠Myodes(早期Cleys onomys)Gallolus特异性地藏匿。它会在人类中引起一种轻度的肾综合征出血热(HFRS)，称为流行性肾病(NE)。NE的临床严重程度因患者而异，并取决于他们的主要组织相容性复合体(MHC)遗传背景。在这项研究中，我们研究了第二类MHC基因多态性在野生水库斑潜蝇对PUUV的敏感性/抵抗力中的潜在作用。我们进行了一项DQA基因外显子2与PUUV抗体之间的关联研究，研究对象是自然种群的银行田鼠。由于免疫基因多态性很可能是由野外的多种寄生虫驱动的，我们还对银行田鼠进行了其他潜在的病毒和寄生虫感染的筛查。我们使用多变量分析来探索DQA多态/PUUV之间的关联，同时考虑整个寄生虫群落的潜在拮抗和/或协同效应。我们的研究表明，第二类MHC特征与包括PUUV和牛痘病毒在内的病毒感染之间存在联系。几个等位基因可能与银行田鼠对这些感染的易感性或抵抗力有关。或者，杂合性似乎与PUUV或任何其他寄生虫感染无关。因此，这一结果没有提供支持通过超显性选择的假说的证据。最后，这种多变量方法揭示了体外寄生虫和PUUV之间的强烈拮抗作用，表明这些寄生虫感染之间存在直接或间接的免疫遗传联系。现在需要其他数据集来确认这些结果，并测试关联是否在空间和/或时间上变化。(C)2007 Elsevier B.V.保留所有权利。
Puumala virus (PUUV) is a hantavirus specifically harboured by the bank vole, Myodes (earlier Clethrionomys) glareolus. It causes a mild form of hemorrhagic fever with renal syndrome (HFRS) in humans, called Nephropathia epidemica (NE). The clinical severity of NE is variable among patients and depends on their major histocompatibility complex (MHC) genetic background. In this study we investigated the potential role of class II MHC gene polymorphism in the susceptibility/resistance to PUUV in the wild reservoir M. glareolus. We performed an association study between the exon 2 of the DQA gene and PUUV antibodies considering a natural population of bank voles. Because immune gene polymorphism is likely to be driven by multiple parasites in the wild, we also screened bank voles for other potential viral and parasitic infections. We used multivariate analyses to explore DQA polymorphism/PUUV associations while considering the potential antagonist and/or synergistic effects of the whole parasite community. Our study suggests links between class II MHC characteristics and viral infections including PUUV and Cowpox virus. Several alleles are likely to be involved in the susceptibility or in the resistance of bank voles to these infections. Alternatively, heterozygosity does not seem to be associated with PUUV or any other parasite infections. This result thus provides no evidence in favour of the hypothesis of selection through overdominance. Finally this multivariate approach reveals a strong antagonism between ectoparasitic mites and PUUV, suggesting direct or indirect immunogenetic links between infections by these parasites. Other datasets are now required to confirm these results and to test whether the associations vary in space and/or time. (C) 2007 Elsevier B.V. All rights reserved.