Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma.
Skp2 expression unfavorably impacts survival in resectable esophageal squamous cell carcinoma.
复制标题
Skp2 表达对可切除食管鳞状细胞癌的生存产生不利影响。
DOI：
10.1186/1479-5876-10-73
复制
发表时间：
2012-05-18
影响因子：
7.4
通讯作者：
Yang HX
中科院分区：
文献类型：
--
作者：
Liang Y;Hou X;Cui Q;Kang TB;Fu JH;Zhang LJ;Luo RZ;He JH;Zeng YX;Yang HX
S相激酶相关蛋白2(Skp2)与食管鳞癌转移和预后的关系尚存争议。本研究的目的是探讨免疫组织化学方法检测Skp2的表达是否与可手术的ESCC患者的临床预后有关，并进一步探讨Skp2影响患者生存的可能机制。包括157个手术切除的ESCC标本的组织微阵列被成功地生成用于免疫组织化学评估。分析Skp2表达的临床/预后意义。术后生存率比较采用Kaplan-Meier分析。使用COX比例风险模型评估临床病理变量和Skp2表达对预后的影响。用细胞增殖实验和集落形成实验检测Skp2在体外对ESCC进展的作用。Skp2的表达与T分期(p = 0.035)和肿瘤病理转移分期(p = 0.027)密切相关。在可切除的鳞状细胞癌中，Skp2的高表达与总体生存不良有关(p = 0.01)。多因素COX回归分析显示，病理T、病理N、细胞分化、Skp2表达阴性是影响总生存率的独立因素。体外培养的ESCC细胞分析表明，Skp2促进了ESCCs的增殖和集落形成能力。在初次切除的食管鳞癌中，Skp2的表达阴性是提高生存率的一个独立因素。Skp2可能在ESCC细胞中起促增殖作用。
The correlation of S-phase kinase–associated protein 2 (Skp2) with metastasis and prognosis in esophageal squamous cell carcinoma (ESCC) is controversial. The purpose of this study was to explore whether there was a correlation between the expression of Skp2 evaluated by immunohistochemistry and the clinical outcome of patients with operable ESCC, and to further determine the possible mechanism of the impact of Skp2 on survival. Tissue microarrays that included 157 surgically resected ESCC specimens was successfully generated for immunohistochemical evaluation. The clinical/prognostic significance of Skp2 expression was analyzed. Kaplan-Meier analysis was used to compare the postoperative survival between groups. The prognostic impact of clinicopathologic variables and Skp2 expression was evaluated using a Cox proportional hazards model. A cell proliferation assay and a colony formation assay were performed in ESCC cell lines to determine the function of Skp2 on the progression of ESCC in vitro. Skp2 expression correlated closely with the T category (p = 0.035) and the pathological tumor-node-metastasis (TNM) stage (p = 0.027). High expression of Skp2 was associated with poor overall survival in resectable ESCC (p = 0.01). The multivariate Cox regression analysis demonstrated that pathological T category, pathological N category, cell differentiation, and negative Skp2 expression were independent factors for better overall survival. In vitro assays of ESCC cell lines demonstrated that Skp2 promoted the proliferative and colony-forming capacity of ESCCs. Negative Skp2 expression in primary resected ESCC is an independent factor for better survival. Skp2 may play a pro-proliferative role in ESCC cells.
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DOI：
10.1186/bcr1278
发表时间：
2005
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Slotky M;Shapira M;Ben-Izhak O;Linn S;Futerman B;Tsalic M;Hershko DD
通讯作者：
Hershko DD
影响因子：
6.2
作者：
Shapira, M;Ben-Izhak, O;Hershko, DD
通讯作者：
Hershko, DD
影响因子：
20.3
作者：
Lim, MS;Adamson, A;Elenitoba-Johnson, KSJ
通讯作者：
Elenitoba-Johnson, KSJ
影响因子：
8
作者：
Radke, S;Pirkmaier, A;Germain, D
通讯作者：
Germain, D
影响因子：
64.8
作者：
Schulman, BA;Carrano, AC;Pavietich, NP
通讯作者：
Pavietich, NP