The effects of non-ionic contrast medium on the hemorheology in vitro and in vivo.
The effects of non-ionic contrast medium on the hemorheology in vitro and in vivo.
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DOI：
10.3233/ch-121669
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发表时间：
2014
影响因子：
2.1
通讯作者：
Jian Song;Xifu Wang;Xiaoli Xu;Binbin Jia;Q. Zheng;W. Ka;Dagong Sun;Z. Wen;Lide Xie;W. Yao
中科院分区：
文献类型：
--
作者：
Jian Song;Xifu Wang;Xiaoli Xu;Binbin Jia;Q. Zheng;W. Ka;Dagong Sun;Z. Wen;Lide Xie;W. Yao
造影剂是放射学中常用的造影剂。然而，由于其高渗透压、高粘度和化学毒性的特性，造影剂的给药已被证明会在短时间内引起不良反应，尤其是对血液流变学的影响。本研究旨在观察非离子型造影剂碘普罗胺对血液流变学的影响。对于体外处理，将人外周血样品与造影剂在37°C下孵育0.5、1和2小时。对于体内研究，将约15 ml造影剂注射到家兔体内，并在推注后0.5、2、6和24 h采集血样。检测血液流变学指标。结果表明，体外造影剂处理后1h红细胞压积调整的全血粘度显著升高，而在推注后0.5h下降，并保持低水平，直至6h。红细胞变形能力在体外2h降至最低，在体内0.5h下降，2h后恢复正常。在体外和体内研究中，造影剂均降低红细胞小变形指数。在体内研究中，红细胞取向指数也降低。在两项研究中，所有时间点的红细胞电泳率均降低，但渗透脆性未发生变化。这些血液流变学指标的异常可能会影响肾脏疾病患者的微循环，引起不良反应。
Contrast media are the commonly used agents in radiology. However, because of their characteristics of high osmolality, high viscosity, and chemical toxicity, the administrations of contrast media have been shown to cause adverse effects especially on hemorheology in short time course. The present study is to find the effects of a non-ionic contrast medium, iopromide, on the hemorheology in long time course both in vitro and in vivo. For in vitro treatment, human peripheral blood samples were incubated with contrast medium at 37°C for 0.5, 1 and 2 h. For in vivo study, about 15 ml of contrast medium was injected into rabbits and blood samples were collected at 0.5, 2, 6, and 24 h after the bolus injection. Hemorheological parameters were examined. Results showed that hematocrit adjusted whole blood viscosity increased significantly at 1 h after in vitro treatment of contrast medium, while it decreased at 0.5 h and remained low till 6 h after bolus injection. Ektacytometer showed that erythrocyte deformability decreased to the lowest level at 2 h in vitro and it dropped at 0.5 h and resumed to normal after 2 h in vivo. Erythrocyte small deformation indices were reduced by contrast medium in both in vitro and in vivo studies. Erythrocyte orientation index was also reduced in in vivo study. Erythrocyte electrophoresis rates at all time points decreased but osmotic fragility did not change in both studies. These impaired hemorheological parameters may disturb the microcirculation and cause adverse effects in patients with kidney diseases.