A chemiluminescent probe for cellular peroxynitrite using a self-immolative oxidative decarbonylation reaction.
A chemiluminescent probe for cellular peroxynitrite using a self-immolative oxidative decarbonylation reaction.
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DOI：
10.1039/c7sc05087a
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发表时间：
2018-03-07
期刊：
影响因子：
8.4
通讯作者：
Lippert AR
中科院分区：
文献类型：
--
作者：
Cao J;An W;Reeves AG;Lippert AR
过氧亚硝酸盐是一种氧化应激的破坏剂，在活细胞中难以监测。本文报道了一种基于靛红的过氧亚硝酸根荧光探针。过氧亚硝酸盐（ONOO-）是一种高度活性的氧物种，已被认为是免疫反应等生理活动的内源性介质，也是病理条件下氧化应激的破坏剂。虽然其生物学重要性越来越清楚，但由于缺乏可用的选择性和灵敏的检测方法，其生产和作用机制的许多细节仍然难以捉摸。在此，我们报告的发展，表征和生物应用的反应为基础的荧光探针ONOO-检测，称为PNCL。PNCL通过氧化脱羰反应与ONOO-通过靛红部分反应，以引发可以立即观察到的光发射，对其他活性硫、氧和氮物质具有高选择性。对靛红与ONOO-的反应进行了详细的研究，证实了ONOO-对NO2-的选择性。PNCL已应用于水溶液和活细胞中ONOO-的检测。此外，PNCL可用于检测在用脂多糖（LPS）刺激以产生免疫应答的巨噬细胞中产生的细胞ONOO。荧光检测的灵敏度以及氧化脱羰反应的特异性为探索ONOO-化学和生物学提供了有用的工具。
Peroxynitrite is a damaging agent of oxidative stress that has been difficult to monitor in living cells. Here, an isatin-based chemiluminescent probe for peroxynitrite is reported. Peroxynitrite (ONOO–) is a highly reactive oxygen species which has been recognized as an endogenous mediator of physiological activities like the immune response as well as a damaging agent of oxidative stress under pathological conditions. While its biological importance is becoming clearer, many of the details of its production and mechanism of action remain elusive due to the lack of available selective and sensitive detection methods. Herein, we report the development, characterization, and biological applications of a reaction-based chemiluminescent probe for ONOO– detection, termed as PNCL. PNCL reacts with ONOO–via an isatin moiety through an oxidative decarbonylation reaction to initiate light emission that can be observed instantly with high selectivity against other reactive sulphur, oxygen, and nitrogen species. Detailed studies were performed to study the reaction between isatin and ONOO–, which confirm selectivity for ONOO– over NO2˙. PNCL has been applied for ONOO– detection in aqueous solution and live cells. Moreover, PNCL can be employed to detect cellular ONOO– generated in macrophages stimulated to mount an immune response with lipopolysaccharide (LPS). The sensitivity granted by chemiluminescent detection together with the specificity of the oxidative decarbonylation reaction provides a useful tool to explore ONOO– chemistry and biology.
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影响因子：
4.8
作者：
Jacobs, AT;Ignarro, LJ
通讯作者：
Ignarro, LJ
影响因子：
4.1
作者：
KOPPENOL, WH;MORENO, JJ;BECKMAN, JS
通讯作者：
BECKMAN, JS
DOI：
10.1152/ajpcell.2000.279.6.c1970
发表时间：
2000-12-01
影响因子：
5.5
作者：
Liu, P;Xu, BH;Wong, PYK
通讯作者：
Wong, PYK
影响因子：
8.4
作者：
Cao J;Lopez R;Thacker JM;Moon JY;Jiang C;Morris SN;Bauer JH;Tao P;Mason RP;Lippert AR
通讯作者：
Lippert AR
一种快速响应、高灵敏度和选择性的荧光探针，用于活细胞中硝酰基的比例成像
影响因子：
4.9
作者：
Liu, Caiyun;Wu, Huifang;Zhang, Xiaoling
通讯作者：
Zhang, Xiaoling