The Lipase Engineering Database: a navigation and analysis tool for protein families
The Lipase Engineering Database: a navigation and analysis tool for protein families
复制标题
DOI：
10.1093/nar/gkg015
复制
发表时间：
2003-01-01
影响因子：
14.9
通讯作者：
Pleiss, J
中科院分区：
文献类型：
--
作者：
Fischer, M;Pleiss, J
脂肪酶工程数据库（LED） （http://www.led.uni-stuttgart.de）整合了关于脂肪酶、酯酶和相关蛋白的序列、结构和功能的信息。806个蛋白的序列数据被分配到38个同源家族中，这些同源家族分为16个超家族，彼此之间没有全局序列相似性。对于每个家族，多序列比对提供了功能相关的注释残基。预先计算的系统发育树允许在超家族内部导航。对45种蛋白质的实验结构进行了叠加和一致的注释。LED已被应用于系统地分析这一庞大而多样的酶类的序列结构和功能关系。这是一个有用的工具来识别除活性位点残基之外的功能相关残基，并设计具有所需底物特异性的突变体。
The Lipase Engineering Database (LED) (http://www.led.uni-stuttgart.de) integrates information on sequence, structure, and function of lipases, esterases, and related proteins. Sequence data on 806 protein entries are assigned to 38 homologous families, which are grouped into 16 superfamilies with no global sequence similarity between each other. For each family, multisequence alignments are provided with functionally relevant residues annotated. Pre-calculated phylogenetic trees allow navigation inside superfamilies. Experimental structures of 45 proteins are superposed and consistently annotated. The LED has been applied to systematically analyze sequence structure function relationships of this vast and diverse enzyme class. It is a useful tool to identify functionally relevant residues apart from the active site residues, and to design mutants with desired substrate specificity.