Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro
Dexamethasone influences human clock gene expression in bronchial epithelium and peripheral blood mononuclear cells in vitro
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DOI：
10.1081/cbi-200062416
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Shimizu, E
中科院分区：
文献类型：
--
作者：
Burioka, N;Takata, M;Shimizu, E
我们确定人外周血单个核细胞（PBMC）是否可以用于分析时钟基因，通过研究它们的mRNA表达在人支气管上皮细胞（BEAS-2B）和PBMCs刺激后的糖皮质激素同系物地塞米松（DEX）在体外。在10：00 h从两名日常活动（类似于07：00至23：00 h）的健康志愿者获得PBMC，并使用真实的时间PCR分析评价体外DEX刺激后hPer 1 mRNA的表达。DEX刺激体外培养的人BEAS-2B细胞和PBMCs后，hPer 1 mRNA表达显著增加。糖皮质激素迅速影响hPer 1 mRNA在PBMC中的表达，这表明人PBMC可能是一个有用的替代标记物，用于研究药物对时钟基因的影响。
We determined whether human peripheral blood mononuclear cells (PBMCs) could be used to analyze clock genes by studying their mRNA expressions in human bronchial epithelium (BEAS-2B) and PBMCs following stimulation by the glucocorticoid homologue dexamethasone (DEX) in vitro. PBMCs were obtained at 10:00 h from two diurnally active (similar to 07:00 to 23:00h) healthy volunteers and were evaluated for hPer1 mRNA expression following DEX stimulation in vitro using real time-PCR analysis. DEX stimulation of human BEAS-2B cells and PBMCs in vitro led to a remarkable increase of hPer1 mRNA. The glucocorticoid rapidly affected the expression of hPer1 mRNA in PBMCs, suggesting that human PBMCs may be a useful surrogate marker for the investigation of drug effects on clock genes.