Bovine blood components: Fractionation, composition, and nutritive value
Bovine blood components: Fractionation, composition, and nutritive value
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DOI：
10.1021/jf9806255
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发表时间：
1999-01-01
影响因子：
6.1
通讯作者：
Sgarbieri, VC
中科院分区：
文献类型：
--
作者：
Duarte, RT;Simoes, MCC;Sgarbieri, VC
本研究工作的目的是将屠宰场采集的牛血破碎成血浆蛋白浓缩物、红细胞浓缩物、球蛋白分离物和羧甲基纤维素-血红素铁（CMC-血红素）复合物。所有四个馏分进行了研究的近似组成和氨基酸和矿物质含量。采用大鼠生物测定法对血浆浓缩蛋白和分离珠蛋白的营养价值进行了比较研究。血浆蛋白浓缩物中氨基酸含量平衡良好，产生的净蛋白利用率和净蛋白率相当于酪蛋白的95%。珠蛋白分离物（类似于91%蛋白质）缺乏异亮氨酸和含S氨基酸，并且在饲料中浓度为10%时无法支持大鼠生长。红细胞浓缩物和分离的CMC-血红素复合物是生物可利用铁的良好来源。CMC-血红素和全血细胞浓缩物的铁利用率（相对于硫酸亚铁为100%）分别为64%和70%。
The objective of this research work was to fractionate bovine blood, collected hygienically in a slaughterhouse, into blood plasma protein concentrate, red blood cell concentrate, globin isolate, and a carboxymethylcellulose-heme iron (CMC-heme) complex. All four fractions were studied for proximate composition and amino acid and mineral contents. The nutritive value of plasma protein concentrate and globin isolate was comparatively studied using rat bioassays. The amino acid content in plasma protein concentrate is well balanced and produced net protein utilization and net protein ratio equivalent to 95% those of casein. Globin isolate (similar to 91% protein) is deficient in isoleucine and S-containing amino acids and was unable to support rat growth at 10% concentration in the diet. Red blood cell concentrate and the isolated CMC-heme complex were good sources of bioavailable iron. Iron availabilities for CMC-heme and whole blood cell concentrate, related to ferrous sulfate as 100%, were 64 and 70%, respectively.