CHARACTERIZATION OF RAT HYPOTHALAMIC CORTICOTROPIN-RELEASING FACTOR
CHARACTERIZATION OF RAT HYPOTHALAMIC CORTICOTROPIN-RELEASING FACTOR
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DOI：
10.1073/pnas.80.15.4851
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
VALE, W
中科院分区：
文献类型：
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作者：
RIVIER, J;SPIESS, J;VALE, W
从大鼠下丘脑提取物中纯化了一种多肽，该多肽具有从培养的大鼠垂体前叶细胞中释放ACTH的高活性和对绵羊下丘脑促肾上腺皮质激素释放因子（CRF）NH_2端（残基4-20）的放射免疫测定活性。根据Edman降解、肽图和氨基酸分析，确定该大鼠CRF的一级结构为：H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH 2。合成大鼠CRF的促垂体效力与分离的天然肽或合成绵羊CRF的效力没有显著偏离。83%的序列同源性表明大鼠和绵羊下丘脑CRF之间的密切结构关系。
A polypeptide was purified from rat hypothalamic extracts on the basis of its high intrinsic activity to release ACTH from cultured rat anterior pituitary cells and its immunoactivity in a radioimmunoassay directed against the NH2 terminus (residues 4-20) of ovine hypothalamic corticotropin-releasing factor (CRF). Based on Edman degradation, peptide mapping and amino acid analysis, the primary structure of this rat CRF was established to be: H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2. The hypophysiotropic potency of synthetic rat CRF did not deviate significantly from the potencies of the isolated native peptide or synthetic ovine CRF. The close structural relationship between rat and ovine hypothalamic CRF is indicated by an 83% sequence homology.