REDUCED SPONTANEOUS ACTIVITY OF MICE DEFECTIVE IN THE EPSILON-4 SUBUNIT OF THE NMDA RECEPTOR-CHANNEL
REDUCED SPONTANEOUS ACTIVITY OF MICE DEFECTIVE IN THE EPSILON-4 SUBUNIT OF THE NMDA RECEPTOR-CHANNEL
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DOI：
10.1016/0169-328x(95)00107-4
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发表时间：
1995-10-01
期刊：
影响因子：
--
通讯作者：
MISHINA, M
中科院分区：
文献类型：
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作者：
IKEDA, K;ARAKI, K;MISHINA, M
为了研究 N-甲基-D-天冬氨酸 (NMDA) 受体通道分子多样性的功能意义，我们通过基因靶向技术产生了 epsilon 4 亚基缺陷的突变小鼠。 epsilon 4亚基突变小鼠生长和交配正常。纯合突变小鼠中未检测到ε 4亚基蛋白，杂合小鼠中155 kDa的ε 4亚基蛋白量减少。其他 NMDA 受体通道亚基 mRNA 的表达并未明显受到突变的影响。突变小鼠的各个大脑区域以及与胡须相关的神经元模式（桶状、桶状和桶状）的形成没有表现出明显的组织学异常。然而，在旷场测试中，epsilon 4 亚基突变小鼠表现出自发活动减少。杂合子小鼠和突变小鼠在运动活动和焦虑测试中没有发现显着差异。这些结果表明NMDA受体通道的ε4亚基在体内控制自发行为活动中发挥作用。
In an attempt to examine the functional significance of the molecular diversity of the N-methyl-D-aspartate (NMDA) receptor channel, we generated mutant mice defective in the epsilon 4 subunit by gene targeting technique. The epsilon 4 subunit mutant mice grew and mated normally. No epsilon 4 subunit protein was detected in the homozygous mutant mice, and the amount of the epsilon 4 subunit protein of 155 kDa was reduced in the heterozygous mice. The expressions of the other NMDA receptor channel subunit mRNAs were not appreciably affected by the mutation. The mutant mice exhibited no obvious histological abnormalities in the various brain regions and in the formation of whisker-related neuronal patterns (barrels, barreloids and barrelettes). In an open field test, however, the epsilon 4 subunit mutant mice showed a reduced spontaneous activity. No significant difference was found between the heterozygous and mutant mice in motor activity and anxiety tests. These results suggest that the epsilon 4 subunit of the NMDA receptor channel plays a role in vivo in controlling the spontaneous behavioral activity.