GOLDEN2-LIKE Transcription Factors Regulate WRKY40 Expression in Response to Abscisic Acid
GOLDEN2-LIKE Transcription Factors Regulate WRKY40 Expression in Response to Abscisic Acid
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GOLDEN2-LIKE 转录因子调节 WRKY40 表达以响应脱落酸
DOI：
10.1104/pp.18.01466
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发表时间：
2019-04-01
期刊：
影响因子：
7.4
通讯作者：
Xu, Zheng-Yi
中科院分区：
文献类型：
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作者：
Ahmad, Rafiq;Liu, Yutong;Xu, Zheng-Yi
拟南芥（Arabidopsis thaliana）GARP（Golden 2、ARR-B、Psr 1）家族转录因子GOLDEN 2-LIKE 1和-2（GLK 1/2）在不同的生物过程中发挥作用;然而，这些转录因子是否以及如何调节对脱落酸（阿坝）的反应仍不清楚。在这项研究中，我们使用了glk 1 glk 2双突变体的阿坝反应中的作用，检查GLK 1/2。glk 1 glk 2双突变体在种子萌发和幼苗发育过程中表现出ABA超敏感表型，在幼苗发育过程中表现出抗渗透胁迫表型。全基因组RNA测序分析的glk 1 glk 2双突变体显示，GLK 1/2调节几个ABA响应基因，包括WRKY 40，在阿坝的存在下。染色质免疫沉淀和凝胶阻滞试验表明，GLK 1/2通过识别共有序列直接与WRKY 40启动子结合。此外，glk 1 glk 2双突变体和wrky 40单突变体的RNA测序分析显示，GLK 1/2和WRKY 40控制一组共同的下游靶基因响应阿坝。此外，遗传互作测试的结果显示，glk 1 glk 2 wrky 40三重突变体对wrky 40单突变体和glk 1 glk 2双突变体显示出类似的阿坝超敏性，而glk 1 glk 2 wrky 40 abi 5-c（ABI 5 CRISPR/Cas9突变体）四重突变体对abi 5 -7单突变体显示出类似的阿坝低敏感性。基于这些结果，我们认为GLK 1/2-WRKY 40转录模块在阿坝反应中起负调控作用。
Arabidopsis (Arabidopsis thaliana) GARP (Golden2, ARR-B, Psr1) family transcription factors, GOLDEN2-LIKE1 and -2 (GLK1/2), function in different biological processes; however, whether and how these transcription factors modulate the response to abscisic acid (ABA) remain unknown. In this study, we used a glk1 glk2 double mutant to examine the role of GLK1/2 in the ABA response. The glk1 glk2 double mutant displayed ABA-hypersensitive phenotypes during seed germination and seedling development and an osmotic stress-resistant phenotype during seedling development. Genome-wide RNA sequencing analysis of the glk1 glk2 double mutant revealed that GLK1/2 regulate several ABA-responsive genes, including WRKY40, in the presence of ABA. Chromatin immunoprecipitation and gel retardation assays showed that GLK1/2 directly associate with the WRKY40 promoter via the recognition of a consensus sequence. Additionally, RNA sequencing analysis of the glk1 glk2 double mutant and wrky40 single mutant revealed that GLK1/2 and WRKY40 control a common set of downstream target genes in response to ABA. Furthermore, results of a genetic interaction test showed that the glk1 glk2 wrky40 triple mutant displayed similar ABA hypersensitivity to the wrky40 single mutant and the glk1 glk2 double mutant, while the glk1 glk2 wrky40 abi5-c (ABI5 CRISPR/Cas9 mutant) quadruple mutant displayed similar ABA hyposensitivity to the abi5-7 single mutant. Based on these results, we propose that the GLK1/2-WRKY40 transcription module plays a negative regulatory role in the ABA response.