Fine mapping and characterisation of a PV-PUR mediating anthocyanin synthesis in snap bean (Phaseolus vulgaris L.)
Fine mapping and characterisation of a PV-PUR mediating anthocyanin synthesis in snap bean (Phaseolus vulgaris L.)
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DOI：
10.1007/s11032-023-01362-8
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发表时间：
2023-03
影响因子：
3.1
通讯作者：
Chang Liu;Xiaoxu Yang;Yongheng He;Qifu Chen;Yucheng Huang;Zhishan Yan;Dajun Liu;Guojun Feng
中科院分区：
文献类型：
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作者：
Chang Liu;Xiaoxu Yang;Yongheng He;Qifu Chen;Yucheng Huang;Zhishan Yan;Dajun Liu;Guojun Feng
花色素苷使菜豆（Phaseolus vulgarisL.）豆荚呈紫色，有助于种子传播，防止环境压力。在这项研究中，我们描述了油豆角紫色的子叶，下胚轴，茎，叶脉，花和荚果组织。突变豆荚中的总花青素、飞燕草素和锦葵色素水平显著高于野生型植株。我们构建了两个群体用于精细定位PV-PURpurple突变基因，该基因位于06号染色体的243.9-kb区域。我们鉴定了编码F3 '5'H的Phvul.006g018800.3为PV-PUR的候选基因。在该基因的编码区发生了6个单碱基突变，改变了蛋白质结构。与野生型相比，T-PV-PUR植株的叶基部和节间为紫色，T-PV-pur植株的表型没有变化，验证了突变基因的功能。结果表明，PV-PUR基因是油豆角花色素苷合成的关键基因，是油豆角产生紫色的主要原因。研究结果为油豆角的育种和改良奠定了基础。
Anthocyanin makes snap bean (Phaseolus vulgarisL.) pods purple, which helps seed dispersal and protects against environmental stress. In this study, we characterised the snap bean purple mutantpv-pur, which has purple cotyledon, hypocotyl, stem, leaf vein, flower and pod tissues. Total anthocyanin, delphinidin and malvidin levels in mutant pods were significantly higher than in wild-type plants. We constructed two populations for fine mapping of thePV-PURpurple mutation gene, located in the 243.9-kb region of chromosome 06. We identifiedPhvul.006g018800.3, encoding F3’5’H, as a candidate gene forPV-PUR. Six single-base mutations occurred in the coding region of this gene, altering protein structure.PV-PURandpv-purgenes were transferred into Arabidopsis, respectively. Compared with the wild-type, the leaf base and internode of T-PV-PUR plant were purple, and the phenotype of T-pv-pur plant remained unchanged, which verified the function of the mutant gene. The results demonstrated thatPV-PURis a crucial gene for anthocyanin biosynthesis in snap bean, resulting in purple colouration. The findings lay a foundation for future breeding and improvement of snap bean.