The effect of unilateral vasectomy on testosterone and testicular parameters in the adult male African giant rat (Cricetomys gambianus)
The effect of unilateral vasectomy on testosterone and testicular parameters in the adult male African giant rat (Cricetomys gambianus)
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DOI：
10.4314/ahs.v13i2.40
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发表时间：
2013-01-01
影响因子：
1
通讯作者：
Azu, O. O.
中科院分区：
文献类型：
--
作者：
Duru, F. I. O.;Ajayi, S.;Azu, O. O.
背景：输精管结扎对精子发生和生殖参数的影响被认为具有物种依赖性，观察到的扰动水平存在显着差异。目的：评估单侧输精管结扎对雄性非洲巨鼠 (AGR)（Cricetomys gambianus）睾酮水平和其他睾丸参数的影响。方法：招募 16 只体重为 500-1300 g 的成年雄性 AGR 进行实验。将动物随机分为三个实验组（1-3）和一个对照组（假手术）组，每组四只大鼠。通过仔细结扎所有实验组（1、2和3）的右侧睾丸的输精管来进行实验性输精管切除术，并且在处死前分别允许动物8、6和2周。假手术动物作为对照。收集血样并检测睾酮，同时进一步处理睾丸组织进行精液和组织病理学分析。结果：生精参数表明实验组和对照组之间精子数量和活力下降的模式以及八周组中的无精症。组织学改变的特点是生精小管萎缩，这与输精管切除术的持续时间成正比。八周时血清睾酮水平显着降低。除第3组外，左右睾丸的精子数量没有统计学上的显着差异。结果表明，AGR的单侧输精管切除术可能对雄性非洲巨鼠的对侧睾丸产生负面影响。结论：这些初步结果表明，AGR的单侧输精管切除术可能会导致睾丸组织结构的紊乱，并可能导致功能下降。
Background: The effects of vasectomy on spermatogenesis and reproductive parameters are recognized to be specie-dependent with marked differences in levels of perturbations observed.Objectives: To assess the impact of unilateral vasectomy on testosterone level and other testicular parameters in the male African giant rat (AGR) (Cricetomys gambianus).Methods: Sixteen adult male AGRs weighing 500-1300 g were recruited for the experiment. Animals were randomly divided into three experimental groups (1-3) and one control (sham operated) group with four rats per group. Experimental vasectomy was done by carefully ligating the vas deferens of the right testis of all the experimental groups (1, 2, and 3) and animals were allowed either 8, 6 and 2 weeks respectively before sacrifice. Sham-operated animals served as the control. Blood samples were collected and assayed for testosterone while testicular tissue was further processed for seminal fluid and histo-pathological analyses.Results: Spermatogenic parameters indicate a pattern of decline in sperm count and motility between the experimental groups and the control and azoospermia in the eight-week group. Histological alterations were marked by atrophy of seminiferous tubules which was proportional to the duration of vasectomy. Serum testosterone levels were significantly reduced at eight weeks. There was no statistically significant difference between sperm counts of right and left testes except for group 3. Results suggest that unilateral vasectomy of the AGR may have negative impact on the contralateral testis in the male African giant rat.Conclusion: These preliminary results reveal that unilateral vasectomy in the AGR may result in perturbations of the histo-architecture of the testes with possible decline in function.