Stimulus properties of venlafaxine in a conditioned taste aversion procedure
Stimulus properties of venlafaxine in a conditioned taste aversion procedure
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DOI：
10.1016/j.ejphar.2008.08.015
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发表时间：
2008-10-31
影响因子：
5
通讯作者：
Uzbay, Tayfun I.
中科院分区：
文献类型：
--
作者：
Kayir, Hakan;Alici, Tevfik;Uzbay, Tayfun I.
文拉法辛的条件刺激特性仍然是未知的。本研究采用条件性味觉厌恶程序考察了文拉法辛的辨别性刺激特性。每天让Swiss韦伯斯特小鼠从2个移液管中接触水20分钟（09：00-11：30 h），加30分钟（15：30 - 16：00 h）。在4天期间，在小鼠第一次饮水后至少1小时，向小鼠注射试验药物[氟西汀、艾司西酞普兰、噻奈普汀、瑞波西汀和N ω-硝基-L-精氨酸甲酯（L-NAME）]。在第5天，它们消耗葡萄糖溶液（5%w/v）并立即注射调节药物（文拉法辛32 mg/kg）。在第8天，允许小鼠在水和葡萄糖溶液之间做出选择。计算每只动物的葡萄糖消耗量占总液体摄入量的百分比。葡萄糖选择的显著减少被定义为条件性味觉厌恶。文拉法辛（32 mg/kg）在小鼠中诱导了强烈的条件性味觉厌恶。预先暴露于噻奈普汀（2.5-10 mg/kg）、氟西汀（10 mg/kg）、艾司西酞普兰（32 mg/kg）和瑞波西汀（5 mg/kg），通过预防文拉法辛诱导的条件性味觉厌恶来替代文拉法辛。L-NAME不能替代文拉法辛。用氟西汀、噻奈普汀、艾司西酞普兰和瑞波西汀替代文拉法辛，进一步证明5-HT和去甲肾上腺素再摄取抑制可能在文拉法辛的刺激效应中起重要作用。(C)2008 Elsevier B. V.保留所有权利。
Conditioned stimulus properties of venlafaxine are still unknown. In the present study, the discriminative stimulus properties of venlafaxine by using a conditioned taste aversion procedure were investigated. Swiss Webster mice were allowed to reach water from 2 pipettes for 20 min (09:00-11:30 h), plus 30 min (15:3016:00 h), daily. During the 4 days, the test drugs [fluoxetine, escitalopram, tianeptine, reboxetine, and N omega-nitro-L-arginine methyl ester(L-NAME)l were injected to mice at least 1 h after they had first water session. On day 5, they consumed glucose solution(5%w/v) and immediately injected with conditioning drug (venlafaxine 32 mg/kg). On day 8, mice were allowed to make a choice between water and glucose solution. The amount of glucose consumption as a percentage of total fluid intakes was calculated for each animal. Significant reduction in glucose choice was defined as conditioned taste aversion. Venlafaxine (32 mg/kg) induced a robust conditioned taste aversion in mice. Pre-exposure to tianeptine (2.5-10 mg/kg), fluoxetine (10 mg/kg), escitalopram (32 mg/kg), and reboxetine (5 mg/kg) substituted for venlafaxine by preventing the conditioned taste aversion induced by venlafaxine. L-NAME did not substitute for venlafaxine. Substitution of venlafaxine by fluoxetine, tianeptine, escitalopram, and reboxetine provides further evidence that both 5-HT and noradrenaline reuptake inhibition may play an important role in the stimulus effect of venlafaxine. (C) 2008 Elsevier B.V. All rights reserved.