The bof gene of bacteriophage P1: DNA sequence and evidence for roles in regulation of phage c1 and ref genes.
The bof gene of bacteriophage P1: DNA sequence and evidence for roles in regulation of phage c1 and ref genes.
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噬菌体 P1 的 bof 基因：DNA 序列以及噬菌体 c1 和 ref 基因调节作用的证据。
DOI：
10.1128/jb.172.6.3269-3277.1990
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发表时间：
1990
影响因子：
3.2
通讯作者：
Hays,JB
中科院分区：
文献类型：
--
作者：
Schaefer,TS;Hays,JB
噬菌体P1的C1阻遏物通过14个或更多个不同的操纵子起作用。这种阻遏物抑制其自身的合成以及其他基因产物的合成。以前，辅助调节基因bof的突变已经显示出增加一些C1调节的P1基因的表达（例如，ref）而是减少其它的表达（例如，禁止）。在这项研究中，bof基因被分离的基础上，它的能力，抑制刺激大肠杆菌染色体重组的P1参考基因，当且仅当一个来源的C1存在。单独使用C1，而不是单独使用Bof，是部分有效的。bofDNA序列编码82个密码子的阅读框，其以TTG密码子开始并包括bof-1（Am）突变和bof：：Tn 5无效突变的位点。ref：：lacZ和cl：：lacZ融合基因的表达被P1 bof-1前噬菌体或质粒编码的C1单独反式部分抑制，这与Ref刺激重组的影响和先前C1自身调节的间接证据一致。质粒编码的C1加Bof或P1 bof+原噬菌体对两种融合基因的抑制更完全。当C1来源还包括编码coi基因的C1上游的0.7-乙酰化酶区域时，单独的C1或C1加Bof对c1：：lacZ和ref：：lacZ的抑制效果要差得多，就好像Coi干扰了C1阻遏物功能一样。
The C1 repressor of bacteriophage P1 acts via 14 or more distinct operators. This repressor represses its own synthesis as well as the synthesis of other gene products. Previously, mutation of an auxiliary regulatory gene, bof, has been shown to increase expression of some C1-regulated P1 genes (e.g., ref) but to decrease expression of others (e.g., ban). In this study the bof gene was isolated on the basis of its ability to depress stimulation of Escherichia coli chromosomal recombination by the P1 ref gene, if and only if a source of C1 was present. C1 alone, but not Bof alone, was partially effective. The bofDNA sequence encodes an 82-codon reading frame that begins with a TTG codon and includes the sites of the bof-1(Am) mutation and a bof::Tn5 null mutation. Expression of ref::lacZ and cl::lacZ fusion genes was partially repressed in trans by a P1 bof-1 prophage or by plasmid-encoded C1 alone, which was in agreement with effects on Ref-stimulated recombination and with previous indirect evidence for c1 autoregulation. Repression of both fusion genes by plasmid-encoded C1 plus Bof or by a P1 bof+ prophage was more complete. When the C1 source also included a 0.7-kilobase region upstream from C1 which encodes the coi gene, repression of both c1::lacZ and ref::lacZ by C1 alone or by C1 plus Bof was much less effective, as if Coi interfered with C1 repressor function.