Smartphone quantifies Salmonella from paper microfluidics
Smartphone quantifies Salmonella from paper microfluidics
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DOI：
10.1039/c3lc50976a
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Yoon, Jeong-Yeol
中科院分区：
文献类型：
--
作者：
Park, Tu San;Li, Wenyue;Yoon, Jeong-Yeol
基于智能手机的光学检测是一种潜在的易于使用的手持式真正的护理点诊断工具，用于早期和快速检测病原体。纸微流体是传统微流体装置的一种低成本、可现场部署且易于使用的替代品。大多数纸基微流体检测通常利用染料或酶-底物结合，而纸质微流体上的细菌检测很少见。我们展示了基于智能手机的沙门氏菌检测在纸微流体上的新颖应用。每个纸质微流体通道均预载有抗鼠伤寒沙门氏菌和抗大肠杆菌结合的亚微粒。将纸质微流体装置浸入沙门氏菌溶液中，导致抗体缀合的颗粒仍被限制在纸纤维内进行免疫凝集。通过评估用智能手机以优化的角度和距离拍摄的数字图像的米氏散射来量化免疫凝集的程度。设计和编程了智能手机应用程序，允许用户将智能手机定位在距纸质微流体装置的最佳角度和距离，并实施简单的图像处理算法来计算和显示智能手机上的细菌浓度。检测限为单细胞水平，总检测时间不到一分钟。
Smartphone-based optical detection is a potentially easy-to-use, handheld, true point-of-care diagnostic tool for the early and rapid detection of pathogens. Paper microfluidics is a low-cost, field-deployable, and easy-to-use alternative to conventional microfluidic devices. Most paper-based microfluidic assays typically utilize dyes or enzyme-substrate binding, while bacterial detection on paper microfluidics is rare. We demonstrate a novel application of smartphone-based detection of Salmonella on paper microfluidics. Each paper microfluidic channel was pre-loaded with anti-Salmonella Typhimurium and anti-Escherichia coli conjugated submicroparticles. Dipping the paper microfluidic device into the Salmonella solutions led to the antibody-conjugated particles that were still confined within the paper fibers to immunoagglutinate. The extent of immunoagglutination was quantified by evaluating Mie scattering from the digital images taken at an optimized angle and distance with a smartphone. A smartphone application was designed and programmed to allow the user to position the smartphone at an optimized angle and distance from the paper microfluidic device, and a simple image processing algorithm was implemented to calculate and display the bacterial concentration on the smartphone. The detection limit was single-cell-level and the total assay time was less than one minute.