The monoclonality of human B-cell lymphomas
The monoclonality of human B-cell lymphomas
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人类 B 细胞淋巴瘤的单克隆性
DOI：
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发表时间：
1977
影响因子：
15.3
通讯作者：
J. Haimovich
中科院分区：
文献类型：
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作者：
R. Levy;R. Warnke;R. Dorfman;J. Haimovich
已通过涉及使用混合抗κ和抗λ抗体的双标记免疫荧光的技术在冷冻切片中检查了涉及淋巴瘤的人类组织中的免疫球蛋白携带细胞。使用纯化抗体的F（ab '）2片段以避免通过Fc受体的任何结合。显示B细胞淋巴瘤由IG承载细胞的单克隆群体组成，而正常或反应性淋巴滤泡包含来自多个克隆的Ig承载细胞的镶嵌。淋巴瘤结节周围常可见正常多克隆B细胞群。我们预期，这里描述的方法将是有用的淋巴瘤的诊断，区分它从反应性淋巴增生的demonstration的单克隆。此外，它应该提供一个敏感和可靠的工具，研究人类淋巴瘤的免疫生物学。
Human tissues involved with lymphoma have been examined in frozen sections for immunoglobulin-bearing cells by a technique involving double-label immunofluorescence with mixed anti-kappa and anti-lambda antibodies. F (ab')2 fragments of purified antibodies were employed to avoid any binding via Fc receptors. B cell lymphomas were shown to be composed of monoclonal populations of Ig bearing cells, whereas normal or reactive lymphoid follicles contained a mosaic of Ig-bearing cells derived from multiple clones. Nodules of lymphoma were often surrounded by normal polyclonal B cell populations. We anticipates that the approach described here will be useful in the diagnosis of lymphoma, differentiating it from reactive lymphoid hyperplasia by the demostration of monoclonality. In addition, it should provide a sensitive and reliable tool for investigating the immunobiology of human lymphoma.