Expression of liver-type fatty-acid-binding protein, fatty acid synthase and vascular endothelial growth factor in human lung carcinoma
Expression of liver-type fatty-acid-binding protein, fatty acid synthase and vascular endothelial growth factor in human lung carcinoma
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DOI：
10.1159/000089417
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发表时间：
2005-01-01
期刊：
影响因子：
5
通讯作者：
Suzuki, T
中科院分区：
文献类型：
--
作者：
Kawamura, T;Kanno, R;Suzuki, T
目的：脂肪酸合成的关键酶脂肪酸合酶（FAS）在包括肺鳞状细胞癌在内的人类癌症中表达，长链脂肪酸通过脂肪酸结合蛋白（FABP）在细胞内运输和/或从血液中摄取。由于在胃腺癌中检测到了 FABP 的一员肝型 (L-) FABP，因此研究了人肺癌中 FAS、L-FABP 和血管内皮生长因子 (VEGF) 的表达，以阐明癌症中脂肪酸的产生和运输机制。方法：采用免疫组化方法检测199例手术切除的肺癌组织中L-FABP、FAS和VEGF的表达。分析了每种蛋白的表达与主要临床病理因素的可能关联。结果：L-FABP 在 60% 的肺癌病例中检出（199 例中的 120 例）；大细胞癌（80%）和腺鳞癌（83%）的检出率升高，但鳞状细胞癌（47%）和小细胞癌（57%）的检出率较低。 FAS 的总体表达率为 67.3%（199 例中的 134 例），VEGF 的总体表达率为 86.8%（199 例中的 158 例）。 L-FABP的表达与FAS状态不相关，但有L-FABP和VEGF共表达的趋势。 L-FABP、FAS 或 VEGF 表达与临床病理数据之间没有关联。结论：L-FABP、FAS和VEGF在人肺癌中高表达，且L-FABP的表达与VEGF相关，但与FAS无关，提示L-FABP可能参与血液中脂肪酸的摄取。版权所有 (C) 2005 S. Karger AG，巴塞尔。
Objective: A key enzyme of fatty acid synthesis, fatty acid synthase (FAS), is expressed in human cancers, including squamous-cell carcinoma of the lung, and long-chain fatty acids are intracellularly transported and/or taken up from blood by fatty-acid-binding proteins (FABPs). Since the liver-type (L-) FABP, a member of the FABPs, is detected in a subset of gastric adenocarcinomas, the expression of FAS, L-FABP and vascular endothelial growth factor ( VEGF) was investigated in human lung carcinomas to elucidate the mechanisms of production and transportation of fatty acid(s) in cancer. Methods: Expression of L- FABP, FAS and VEGF in 199 surgically resected lung carcinomas was examined immunohistochemically. Possible associations of the expression of each protein with major clinicopathological factors were analyzed. Results: L-FABP was detected in 60% (120 of 199) of the lung carcinoma cases; detection was increased in large-cell carcinoma (80%) and adenosquamous carcinoma (83%), but low in squamous-cell carcinoma (47%) and in small-cell carcinoma (57%). Overall expression of FAS was 67.3% (134 of 199 cases) and that of VEGF was 86.8% (158 of 199 cases), respectively. Expression of L-FABP was not correlated with the FAS status, but there was a tendency to co-expression of L-FABP and VEGF. There was no association between L-FABP, FAS or VEGF expression and clinicopathological data. Conclusions: L-FABP, FAS and VEGF are highly expressed in human lung cancer, and expression of L-FABP is associated with that of VEGF but not that of FAS, suggesting that L-FABP might be involved in the uptake of fatty acid(s) from the bloodstream. Copyright (C) 2005 S. Karger AG, Basel.