Transcription factors in primary glial cultures: Changes with neuronal interactions
Transcription factors in primary glial cultures: Changes with neuronal interactions
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DOI：
10.1016/0169-328x(95)00318-m
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发表时间：
1996-04-01
期刊：
影响因子：
--
通讯作者：
McMillian, MK
中科院分区：
文献类型：
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作者：
Pennypacker, KR;Hong, JS;McMillian, MK
几种星形胶质细胞基因产物，如脑啡肽和胶质细胞酸性蛋白（GFAP），在体外条件下相对于体内以更高水平表达。我们已经观察到，培养的神经胶质细胞表达高基础水平的转录因子，如Fos相关抗原（Fra），c-Jun，JunD，和cAMP反应元件结合蛋白（CREB）。当神经元细胞铺在单层的顶部时，星形胶质细胞中Fra、c-Jun、JunD和GFAP的表达降低。在这些培养物中检测了与GFAP和脑啡肽原基因的AP-1样位点的DNA结合活性。从胶质细胞的文化，它承认的GFAP AP-1元素的蛋白复合物含有Fra免疫反应，而从混合神经元/胶质细胞的文化的DNA结合CREB免疫反应蛋白。在神经胶质细胞的文化，没有绑定发生前脑啡肽AP-1样元素，但CREB-免疫反应复合物承认这个序列在混合文化。因此，随着神经元的加入，星形胶质细胞中的转录因子和靶基因产物都减少。组成基因调节复合物的蛋白质也发生变化，表明星形胶质细胞基因表达的调节是由神经元调节的。
Several astrocyte gene products, such as enkephalin and glial fibrillary acidic protein (GFAP), are expressed at higher levels under in vitro conditions relative to in vivo. We have observed that cultured glial cells express high basal levels of transcription factors, such as fos-related antigens (Fra), c-Jun, JunD, and cAMP responsive element binding protein (CREB). When neuronal cells are plated on top of the monolayers, the expression of Fra, c-Jun, JunD, and GFAP decreases in the astroglial cells. The DNA binding activity to the AP-l-Like sites of the GFAP and proenkephalin genes was examined in these cultures. The protein complex from glial cultures which recognizes the GFAP AP-1 element contained Fra immunoreactivity while the DNA binding from mixed neuronal/glial cultures consists of CREB-immunoreactive proteins. In glial cultures, no binding occurred to the proenkephalin AP-1-Iike element but a CREB-immunoreactive complex recognized this sequence in the mixed cultures. Thus, with the addition of neurons, both transcription factors and target gene products decrease in astroglial cells. The proteins that compose gene modulatory complexes also change suggesting that regulation of astroglial gene expression is modulated by neurons.