Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family
Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family
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DOI：
10.1038/sj.cr.7310035
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发表时间：
2006-03-01
期刊：
影响因子：
44.1
通讯作者：
Gu, Hongya
中科院分区：
文献类型：
--
作者：
Guo, Lei;Wang, Zi Yi;Gu, Hongya
质膜内在蛋白（PIP）是水通道蛋白的一个亚家族，能够使水快速和受控地跨膜转运。本研究系统地鉴定并克隆了10个水稻PIP基因。根据它们编码的氨基酸序列的相似性，将这些水稻PIP基因分为两类，并按照玉米PIP基因的命名法将其命名为OsPIP 1 -1至OsPIP 1 -3和OsPIP 2 -1至OsPIP 2 -7。定量RT-PCR分析鉴定了3个根特异性OsPIP基因和1个叶特异性OsPIP基因。此外，每个OsPIP基因的表达谱响应盐，干旱和阿坝处理进行了详细检查。对野生型拟南芥中过量表达OsPIP 1（OsPIP 1 -1）或OsPIP 2（OsPIP 2 -2）的转基因植物的分析显示，对盐（100 mM NaCl）和干旱（200 mM甘露醇）的耐受性增强，但对更高浓度的盐处理（150 mM NaCl）没有耐受性。综上所述，这些数据表明每个OsPIP基因在响应不同的胁迫中具有不同的作用，并且应该为理解水稻PIP基因的生理功能增加新的层次。
Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporins that enable fast and controlled translocation of water across the membrane. In this study, we systematically identified and cloned ten PIP genes from rice. Based on the similarity of the amino acid sequences they encoded, these rice PIP genes were classified into two groups and designated as OsPIP1-1 to OsPIP1-3 and OsPIP2-1 to OsPIP2-7 following the nomenclature of PIP genes in maize. Quantitative RT-PCR analysis identified three root-specific and one leaf-specific OsPIP genes. Furthermore, the expression profile of each OsPIP gene in response to salt, drought and ABA treatment was examined in detail. Analysis on transgenic plants over-expressing of either OsPIP1 (OsPIP1-1) or OsPIP2 (OsPIP2-2) in wild-type Arabidopsis, showed enhanced tolerance to salt (100 mM of NaCl) and drought (200 mM of mannitol), but not to salt treatment of higher concentration (150 mM of NaCI). Taken together, these data suggest a distinct role of each OsPIP gene in response to different stresses, and should add a new layer to the understanding of the physiological function of rice PIP genes.