Complete genome sequences of hepatitis C virus subtype 3i and 3a subtype isolates from India
Complete genome sequences of hepatitis C virus subtype 3i and 3a subtype isolates from India
复制标题
DOI：
10.1007/s12664-010-0030-3
复制
发表时间：
2010-05-01
影响因子：
2
通讯作者：
Arankalle, V.
中科院分区：
文献类型：
--
作者：
Gupte, Gouri;Ramdasi, Ashwini;Arankalle, V.
目的丙型肝炎病毒(简称丙型肝炎)是慢性肝炎的主要病原体，主要分为6种主要基因型别。3型丙型肝炎病毒感染对干扰素治疗更敏感。在印度，3型，特别是3a亚型的丙型肝炎病毒感染很常见。根据部分基因组序列，从印度鉴定出三种新的丙型肝炎病毒亚型，即3G、3J和3I。本报告提供了3i和3a亚型各1株的全基因组序列。结果印度分离的丙型肝炎病毒3i株全基因组序列与其他3型序列聚在一起，与3b和3a亚型更接近(核苷酸同源性分别为80.5%和79.1%[SD 0.4%])。核苷酸相似性在核心区最高(86.1-88.7%)，在E2区最低(69.4-70.7%)。系统发育树分析证实存在一个独立的亚型3i。印度丙型肝炎病毒3a分离株的全基因组序列与已知的3a型序列聚在一起，核苷酸相似性为91.1%(SD 0.2%)。结论获得的3型丙型肝炎病毒分离株全基因组序列信息将有助于进一步研究丙型肝炎病毒的进化和分型，并为制定新的治疗和预防措施提供依据。
Aim Hepatitis C virus (HCV), a major causative agent of chronic hepatitis, is classified into six major genotypes. Genotype 3 HCV infection is more sensitive to interferon therapy. In India, genotype 3, particularly subtype 3a, HCV infections are common. Three novel HCV subtypes i.e., 3g, 3j, and 3i were identified from India based on partial genomic sequences. This report provides full genome sequences of one isolate each of subtypes 3i and 3a.Methods Serum samples positive for subtype 3i and 3a HCV RNA based on core region genomic sequences were studied. Complete HCV genomes were amplified as 11 overlapping PCR fragments and sequenced.Results The complete genomic sequence of Indian HCV 3i isolate clustered with other genotype 3 sequences, and was closer to subtypes 3b and 3a (80.5% and 79.1% [SD 0.4%] nucleotide identity). Nucleotide similarities were the highest in the core region (86.1-88.7%), and the least in the E2 region (69.4-70.7%). Phylogenetic tree analysis confirmed the existence of a separate subtype 3i. The Indian HCV 3a isolate's complete genomic sequences clustered with previously known genotype 3a sequences with a nucleotide similarity of 91.1% (SD 0.2%). Neither isolates showed evidence of recombination of different HCV genotypes.Conclusion The information on complete genomic sequences of the genotype 3 HCV isolates should be helpful in future studies on HCV evolution and classification, and for development of newer therapeutic and preventive strategies against this infection.