Differentiation, evolution and utilization of natural alleles for cold adaptability at the reproductive stage in rice.
Differentiation, evolution and utilization of natural alleles for cold adaptability at the reproductive stage in rice.
复制标题
水稻生殖期冷适应天然等位基因的分化、进化与利用
DOI：
10.1111/pbi.13424
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发表时间：
2020-12
影响因子：
13.8
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Guo H;Zeng Y;Li J;Ma X;Zhang Z;Lou Q;Li J;Gu Y;Zhang H;Li J;Li Z
摘要水稻生殖阶段耐冷性的遗传研究可显著减少产量损失。然而，知识的遗传基础和适应性分化，以及潜在的自然等位基因的进化和利用，仍然有限。本研究利用两个关联小组的580份水稻材料进行全基因组关联研究，共鉴定出156个与生殖期耐冷性相关的基因座。Os 01 g 0923600和Os 01 g 0923800被鉴定为qCTB 1 t的主要相关基因。通过群体遗传分析，分别确定了22个和29个控制亚种间（籼/籼和粳/粳）和粳内冷适应分化的趋异区域。对克隆的4个耐寒基因的联合分析表明，它们在不同的气候条件下有不同的来源和利用。bZIP 73和OsAPX 1区分亚种间直接从野生稻进化而来，而新突变CTB 4a和Ctb 1在耿适应寒冷气候期间出现。在驯化和育种过程中，耐寒的耿种质在较冷的气候条件下经历了比其他种质更强的选择。四个已鉴定基因的显性等位变异体的加性效应在现代水稻品种的冷适应中起重要作用。本研究为进一步发掘水稻生殖期耐冷性基因和改良水稻生殖期耐冷性育种提供了有价值的信息。
Abstract Genetic studies on cold tolerance at the reproductive stage in rice could lead to significant reductions in yield losses. However, knowledge about the genetic basis and adaptive differentiation, as well as the evolution and utilization of the underlying natural alleles, remains limited. Here, 580 rice accessions in two association panels were used to perform genome‐wide association study, and 156 loci associated with cold tolerance at the reproductive stage were identified. Os01g0923600 and Os01g0923800 were identified as promising candidate genes in qCTB1t, a major associated locus. Through population genetic analyses, 22 and 29 divergent regions controlling cold adaptive differentiation inter‐subspecies (Xian/Indica and Geng/Japonica) and intra‐Geng, respectively, were identified. Joint analyses of four cloned cold‐tolerance genes showed that they had different origins and utilizations under various climatic conditions. bZIP73 and OsAPX1 differentiating inter‐subspecies evolved directly from wild rice, whereas the novel mutations CTB4a and Ctb1 arose in Geng during adaptation to colder climates. The cold‐tolerant Geng accessions have undergone stronger selection under colder climate conditions than other accessions during the domestication and breeding processes. Additive effects of dominant allelic variants of four identified genes have been important in adaptation to cold in modern rice varieties. Therefore, this study provides valuable information for further gene discovery and pyramiding breeding to improve cold tolerance at the reproductive stage in rice.