[Effect of electroacupuncture at "Jiaji" (EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury].
[Effect of electroacupuncture at "Jiaji" (EX-B 2) points combined with nerve mobilization on motor function and mRNA and protein expression of RhoA in rabbits with sciatic nerve injury].
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DOI：
10.13703/j.0255-2930.2019.06.016
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发表时间：
2019-06-12
期刊：
影响因子：
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通讯作者：
Yuan, Yi-Ming
中科院分区：
文献类型：
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作者：
Wang, Yan;Dong, Chuan-Fei;Yuan, Yi-Ming
目的：观察电针夹脊（EX-B 2）穴联合神经松动术对坐骨神经损伤家兔RhoA蛋白及mRNA表达的影响，为电针“夹脊”（EX-B 2）穴联合神经松动术治疗周围神经损伤提供理论依据。 方法：将180只新西兰兔随机分为正常组和正常组。对照组、模型对照组、神经松动组、电针组、电针加神经松动组，每组36只。每组又分为1周亚组、2周亚组和4周亚组，每个亚组12只。采用夹紧法制作坐骨神经损伤模型。正常对照组的兔子不接受任何干预。模型对照组兔术后正常饲养。神经松动组给予神经松动治疗；手法持续1秒，放松5秒，每天10次，每周6天。电针组采用电针治疗“夹脊”(EX-B 2)穴(L4-L6),每日1次,每次30 min,每周6次。电针加神经松动组采用电针治疗“夹脊”(EX-B 2)穴位，然后进行神经松动。采用脚趾张力反射和改良Tarlov评分评价伤侧坐骨神经功能；取脊髓L4-L6及坐骨神经相应节段的组织。实时荧光定量PCR检测RhoA基因的表达，Western Blot检测RhoA蛋白的表达。 结果：①足趾张力反射及改良Tarlov评分：模型对照组第1、2、4周评分均低于正常对照组（均P）。
OBJECTIVE: To observe the effects of electroacupuncture (EA) at "Jiaji" (EX-B 2) points combined with nerve mobilization on protein and mRNA expression of RhoA in rabbits with sciatic nerve injury, and to provide theoretical basis for the treatment of peripheral nerve injury by EA at "Jiaji" (EX-B 2) points combined with nerve mobilization.METHODS: A total of 180 New Zealand rabbits were randomly divided into a normal control group, a model control group, a nerve mobilization group, an EA group, an EA plus nerve mobilization group, 36 rabbits in each group. Each group was further divided into a 1-week subgroup, 2-week subgroup and 4-week subgroup, 12 rabbits in each subgroup. The sciatic nerve injury model was made by clamping method. The rabbits in the normal control group did not receive any intervention. The rabbits in the model control group was normally fed after operation. The rabbits in the nerve mobilization group were treated with nerve mobilization; the manipulation lasted for 1 s and relaxed for 5 s, 10 times per day, 6 days per week. The rabbits in the EA group were treated with EA at "Jiaji" (EX-B 2) points (L4-L6), once a day, 30 min each time, 6 times per week. The rabbits in the EA plus nerve mobilization group were treated with EA at "Jiaji" (EX-B 2) points, followed by nerve mobilization. The function of sciatic nerve on the injured side was evaluated by toe tension reflex and modified Tarlov score; the tissues of corresponding segments of spinal cord L4-L6 and sciatic nerve were taken; the expression of RhoA gene was detected by real-time PCR and the expression of RhoA protein was detected by Western Blot.RESULTS: ① Toe tension reflex and modified Tarlov score: at 1, 2 and 4 weeks, the scores in the model control group were lower than those in the normal control group (all P