Detection of Lion Podoplanin Using the Antitiger Podoplanin Monoclonal Antibody PMab-231
Detection of Lion Podoplanin Using the Antitiger Podoplanin Monoclonal Antibody PMab-231
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使用抗虎 Podoplanin 单克隆抗体 PMab-231 检测 Lion Podoplanin
DOI：
10.1089/mab.2020.0008
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发表时间：
2020
影响因子：
--
通讯作者：
Kaneko Mika K.
中科院分区：
文献类型：
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作者：
Kato Yukinari;Takei Junko;Sano Masato;Asano Teizo;Sayama Yusuke;Uchida Kazuyuki;Nakagawa Takayuki;Kaneko Mika K.
免疫组化分析需要特异性靶向podoplanin（PDPN）（一种I型肺泡细胞的标志物）的单克隆抗体（mAb）。抗PDPN mAb可用于许多物种，包括人、小鼠、大鼠、兔、狗、猫、牛、猪、袋獾、羊驼、老虎、鲸鱼、山羊、马、熊和绵羊PDPN。然而，尚未开发出抗狮子PDPN（lioPDPN）抗体。在本研究中，检查了可用抗PDPN mAb和lioPDPN之间可能的交叉反应。免疫组化结果显示，抗虎PDPN单克隆抗体PMab-231（IgG 2a，kappa）与狮肺I型肺泡细胞反应，提示PMab-231可用于检测lioPDPN。
Monoclonal antibodies (mAbs) that specifically target podoplanin (PDPN), a marker for type I alveolar cells, are needed for immunohistochemical analyses. Anti-PDPN mAbs are available for many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, Tasmanian devil, alpaca, tiger, whale, goat, horse, bear, and sheep PDPNs. However, no antilion PDPN (lioPDPN) antibody has been developed. In this study, possible cross-reaction between available anti-PDPN mAbs and lioPDPN was examined. Immunohistochemical analysis showed that antitiger PDPN mAb PMab-231 (IgG2a, kappa) reacted with type I alveolar cells from lion lung, indicating that PMab-231 is useful for the detection of lioPDPN.