The cauliflower mosaic virus virion-associated protein is dispensable for viral replication in single cells
The cauliflower mosaic virus virion-associated protein is dispensable for viral replication in single cells
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DOI：
10.1128/jvi.76.18.9457-9464.2002
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发表时间：
2002-09-01
影响因子：
5.4
通讯作者：
Hohn, T
中科院分区：
文献类型：
--
作者：
Kobayashi, K;Tsuge, S;Hohn, T
花椰菜花叶病毒（CaMV）开放阅读框III（ORF III）编码病毒体相关蛋白（Vap），其是蚜虫传播所必需的两种病毒蛋白之一。然而，与由CaMV ORF II编码的蚜虫传播因子不同，Vap也是系统感染所必需的，这表明它是一种多功能蛋白。为了阐明Vap的额外功能或功能，我们测试了转染芜菁原生质体中非感染性ORF III缺陷突变体的复制。PCR和Western印迹分析表明，CaMV复制发生的效率类似于野生型病毒，而不会导致逆转。电子显微镜检查显示ORF III移码突变体形成正常结构的病毒体。这些结果表明，Vap在单细胞中复制是必需的，并且对于病毒体形态发生不是必需的。对接种的芜菁叶的分析表明ORF III移码突变体不会引起任何可检测的局部感染。这些结果强烈表明Vap在病毒移动中的作用。
Cauliflower mosaic virus (CaMV) open reading frame III (ORF III) codes for a virion-associated protein (Vap), which is one of two viral proteins essential for aphid transmission. However, unlike the aphid transmission factor encoded by CaMV ORF II, Vap is also essential for systemic infection, suggesting that it is a multifunctional protein. To elucidate the additional function or functions of Vap, we tested the replication of noninfectious ORF III-defective mutants in transfected turnip protoplasts. PCR and Western blot analyses revealed that CaMV replication had occurred with an efficiency similar to that of wild-type virus and without leading to reversions. Electron microscopic examination revealed that an ORF III frameshift mutant formed normally structured virions. These results demonstrate that Vap is dispensable for replication in single cells and is not essential for virion morphogenesis. Analysis of inoculated turnip leaves showed that the ORF III frameshift mutant does not cause any detectable local infection. These results are strongly indicative of a role for Vap in virus movement.