Jα33+ MAIT cells play a protective role in TNBS induced intestinal inflammation.
Jα33+ MAIT cells play a protective role in TNBS induced intestinal inflammation.
复制标题
Jα33+ MAIT 细胞在 TNBS 诱导的肠道炎症中发挥保护作用。
作者：
Ruijin Xiao;Mengjun Wu;Xiaoling Zheng;Peng Shu;Wang Mei;Yingcheng Zheng;Yuling He;Jinquan Tan
背景/目标
肠粘膜中选择性聚集的粘膜相关T淋巴细胞(MAIT)细胞可能参与免疫调节。MAIT细胞由其Vα7.2-Jα33(人)/Vα19-Jα33(小鼠)不变链决定。人Jα33和小鼠Jα33的编码序列完全相同。为了研究MAIT细胞在IBD中的作用，我们制备了抗Jα33抗体，用来检测MAIT细胞在三七总皂甙诱导的IBD模型中的作用。
方法论
用TNBS诱导雄性BALB/c小鼠结肠炎。将正常小鼠的J-α-33+MAIT细胞作为供体转移到TnBS诱导的小鼠体内。在转移Jα33+MAIT细胞的条件下，对结肠炎的严重程度进行临床和组织学评估。
结果
给药后大鼠结肠粘膜中MAIT-aTCRmRNA和蛋白表达水平降低，J-α-33+-MAIT细胞聚集在脾内。应用TNBS后，疾病活动指数(DAI)增加，DAI由体重减轻、大便稠度和肠道出血决定。转移的Jα33+MAIT细胞显著降低疾病活动性指数评分的增加。
结论
综上所述，上述结果表明，肠粘膜中Jα33+MAIT细胞的聚集改善了肠炎的发生。结论：Jα33+MAIT细胞对肿瘤坏死因子诱导的肠道炎症具有保护作用。
BACKGROUND/AIMS
Mucosa-associated T-lymphocyte (MAIT) cells that are selectively accumulated in the intestinal mucosa may be involved in immune regulation. MAIT cells are determined by their Vα7.2-Jα33 (human)/Vα19-Jα33 (mice) invariant chain. The encoding DNA sequences of Human Jα33 and mouse Jα33 are identical. In order to study the role of MAIT cells in IBD we produced anti-Jα33 antibody to detect MAIT cells in TNBS induced IBD models.
METHODOLOGY
Colitis was induced by TNBS in male BALB/c mice. Jα33+MAIT cells from normal mice were transferred into TNBS induced mice as donors. Colitis severity was evaluated clinically and histologically, under the condition of transferred Jα33+MAIT cells.
RESULTS
The mRNA and protein expression levels of MAIT aTCR in the colonic mucosa were decreased after TNBS administration; Jα33+MAIT cells were aggregated in spleen after TNBS administration. The disease activity index (DAI) which was determined by weight loss, stool consistency and intestinal bleeding, increased after TNBS administration. Transferred Jα33+MAIT cells significantly degrade the increase in the disease activity index scores.
CONCLUSIONS
Taken together, the results indicated that the aggregation of Jα33+MAIT cells in intestinal mucosa improved TNBS-induced colitis. We conclude that Jα33+MAIT cells play a protective role in TNBS induced intestinal inflammation.