Identification, expression, and tissue distribution of the three rat lysyl hydroxylase isoforms
Identification, expression, and tissue distribution of the three rat lysyl hydroxylase isoforms
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DOI：
10.1016/s0006-291x(03)01262-2
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发表时间：
2003-08-08
影响因子：
3.1
通讯作者：
Robins, SP
中科院分区：
文献类型：
--
作者：
Mercer, DK;Nicol, PF;Robins, SP
赖氨酰羟化酶（LH）（原胶原-赖氨酸2-酮戊二酸5-双加氧酶; PLOD）在胶原蛋白的翻译后修饰期间催化赖氨酸残基的羟基化。在本文中，我们描述了第一个识别和克隆的LH亚型2和3从大鼠，包括两个LH 2剪接变体（LH 2a和LH 2b）。大鼠LH在几乎所有检查的组织和细胞类型中都表达，这表明LH功能可能缺乏组织特异性。所有LH亚型均稳定转染至CHO-K1细胞中，这代表了在真核细胞系中重组LH生产的第一个实例。所有LH亚型的表达和产生导致总胶原合成增加。LH 1和LH 2a的表达和产生导致总吡啶鎓交联产生的增加。证据表明，LH 2a具有端肽赖氨酰羟化酶活性，以前被认为是一种新的酶，提出。(C)2003年爱思唯尔公司All rights reserved.
Lysyl hydroxylases (LH) (procollagen-lysine 2-oxoglutarate 5-dioxygenase; PLOD) catalyse the hydroxylation of lysine residues during the post-translational modification of collagenous proteins. In this paper, we describe the first identification and cloning of LH isoforms 2 and 3 from the rat, including both LH2 splice variants (LH2a and LH2b). The rat LHs are expressed in almost all tissue and cell types examined, indicating a probable lack of tissue specificity for LH function. All LH isoforms were stably transfected into CHO-K1 cells and this represents the first example of recombinant LH production in a eukaryotic cell line. Expression and production of all LH isoforms led to an increase in total collagen synthesis. LH1 and LH2a expression and production led to an increase in total pyridinium cross-link production. Evidence that LH2a possesses telopeptide lysyl hydroxylase activity, previously thought to be a novel enzyme, is presented. (C) 2003 Elsevier Inc. All rights reserved.