Immunization with baculovirus-expressed VP4 protein passively protects against simian and murine rotavirus challenge
Immunization with baculovirus-expressed VP4 protein passively protects against simian and murine rotavirus challenge
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DOI：
10.1128/jvi.64.4.1698-1703.1990
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发表时间：
1990-04
影响因子：
5.4
通讯作者：
Erich R. Mackow;P. Vo;R. Broome;Dorsey Bass;Harry B. Greenberg
中科院分区：
文献类型：
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作者：
Erich R. Mackow;P. Vo;R. Broome;Dorsey Bass;Harry B. Greenberg
一种杆状病毒表达的VP 4蛋白来源于猿猴恒河猴轮状病毒（RRV）被用来肠胃外免疫小鼠母鼠。VP 4免疫的母鼠产生了高水平的抗RRV中和抗体和低水平的抗人毒株Wa、ST 3和S2以及动物毒株SA-11、NCDV和Eb的交叉反应性中和抗体。用VP 4免疫的母鼠哺乳的新生小鼠可免受猴RRV毒株强毒攻击剂量和鼠轮状病毒Eb的攻击。由VP 4免疫产生的血清中和应答的交叉反应性质和免疫的保护效力表明重组表达的VP 4蛋白应被视为可行的疫苗候选物。
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies against RRV and low levels of cross-reactive neutralizing antibodies against human strains Wa, ST3, and S2 and animal strains SA-11, NCDV, and Eb. Newborn mice suckled on VP4-immunized dams were protected against a virulent challenge dose of the simian strain RRV and against murine rotavirus Eb. The cross-reactive nature of the serum-neutralizing response generated by VP4 immunization and the protective efficacy of the immunization suggest that recombinant-expressed VP4 proteins should be considered as viable vaccine candidates.