Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E-coli isolates from dogs with urinary tract infection
Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E-coli isolates from dogs with urinary tract infection
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DOI：
10.1128/jcm.41.1.337-345.2003
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发表时间：
2003-01-01
影响因子：
9.4
通讯作者：
Ling, GV
中科院分区：
文献类型：
--
作者：
Johnson, JR;Kaster, N;Ling, GV
犬自发性尿路感染（UTI）被用作一种自然实验，以深入了解人类尿路感染的发病机制。对来自37只患有尿路感染的犬的尿液和直肠大肠杆菌分离株在系统发育背景、O抗原和扩展毒力基因型方面进行了比较。在54%的尿路感染病例中，犬的尿液和直肠分离株为同一菌株。尿液分离株与仅直肠分离株有显著差异，因为它们主要来源于大肠杆菌系统发育B2组，表达典型的（人类）与尿路感染相关的O抗原，并拥有许多与毒力相关的基因，最显著的是pap元件（P菌毛）、papG（黏附素）等位基因III、sfa/foc和sfaS（S菌毛）、hly（溶血素）、fyuA（耶尔森杆菌素）、iroN（铁载体）和ompT（外膜蛋白酶T）。根据所分析的标记，与宿主主要直肠菌株相对应的20个尿液分离株的毒力并不低于与宿主主要直肠菌株不同的17个尿液分离株。这些发现表明，犬和人类的尿路感染发病机制相似，为尿路感染发病机制的特殊致病性假说而非流行假说提供了更多支持，并确定了许多特定的与毒力相关的因素是尿毒力的重要相关因素。
Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulence genotype. In 54% of the UTI episodes, the dog's urinary and rectal isolates represented the same strain. Urinary isolates differed dramatically from rectal-only isolates in that they derived predominantly from E. coli phylogenetic group B2, expressed typical (human) UTI-associated O antigens, and possessed many virulence-associated genes, most notably pap elements (P fimbriae), papG (adhesin) allele III, sfa/foc and sfaS (S fimbriae), hly (hemolysin),fyuA (yersiniabactin), iroN (siderophore), and ompT (outer membrane protease T). The 20 urinary isolates that corresponded with the host's predominant rectal strain were no less virulent according to the markers analyzed than were the 17 urinary isolates that differed from the host's predominant rectal strain. These findings suggest that UTI pathogenesis is similar in dogs and humans, provide added support for the special-pathogenicity over the prevalence hypothesis of UTI pathogenesis, and identify numerous specific virulence-associated factors as significant correlates of urovirulence.