Adaptive optics optical coherence tomography at 120,000 depth scans/s for non-invasive cellular phenotyping of the living human retina.
Adaptive optics optical coherence tomography at 120,000 depth scans/s for non-invasive cellular phenotyping of the living human retina.
复制标题
自适应光学相干断层扫描，以120,000的深度扫描/s进行无创的人类视网膜的非侵入性细胞表型。
DOI：
10.1364/oe.17.019382
复制
发表时间：
2009-10-26
期刊：
影响因子：
3.8
通讯作者：
Drexler W
中科院分区：
文献类型：
--
作者：
Torti C;Povazay B;Hofer B;Unterhuber A;Carroll J;Ahnelt PK;Drexler W
本文介绍了一种成功的组合超高速（120，000深度扫描/秒），超高分辨率的光学相干断层扫描与自适应光学和消色差透镜的单色和纵向色差的眼睛像差，分别补偿，允许在细胞分辨率的正常和病理人类视网膜的非侵入性体积成像。该成像系统的能力在这里通过探测细胞视网膜内结构，还没有访问到目前为止，在体内，非侵入性，无标记的成像技术，包括色素上皮细胞，微血管的脉络膜毛细血管，单一的神经纤维束和胶原板的筛板在视神经乳头的初步研究证明。此外，在两个色盲锥损失的体积范围可以量化的第一次。这项新的技术提供了机会，以加强对视网膜发病机制的理解和早期诊断视网膜疾病。
This paper presents a successful combination of ultra-high speed (120,000 depth scans/s), ultra-high resolution optical coherence tomography with adaptive optics and an achromatizing lens for compensation of monochromatic and longitudinal chromatic ocular aberrations, respectively, allowing for non-invasive volumetric imaging in normal and pathologic human retinas at cellular resolution. The capability of this imaging system is demonstrated here through preliminary studies by probing cellular intraretinal structures that have not been accessible so far with in vivo, non-invasive, label-free imaging techniques, including pigment epithelial cells, micro-vasculature of the choriocapillaris, single nerve fibre bundles and collagenous plates of the lamina cribrosa in the optic nerve head. In addition, the volumetric extent of cone loss in two colour-blinds could be quantified for the first time. This novel technique provides opportunities to enhance the understanding of retinal pathogenesis and early diagnosis of retinal diseases.