Muscle actin gene from Aedes aegypti (Diptera: Culicidae)
Muscle actin gene from Aedes aegypti (Diptera: Culicidae)
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DOI：
10.1093/jmedent/33.6.955
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发表时间：
1996-11-01
影响因子：
2.1
通讯作者：
Scott, AL
中科院分区：
文献类型：
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作者：
Ibrahim, MS;Eisinger, SW;Scott, AL
从黄热病蚊子埃及伊蚊（Aedes aegypti（L.））中分离出含有编码肌动蛋白的1，240-bp插入片段的重组噬菌粒，互补DNA文库该插入片段（pBS-Act35）含有一个822 bp的开放阅读框，其推导的氨基酸序列与果蝇（DrosophilamelanogasterMeigen）和家蚕（Bombyxmori（L.）肌动蛋白基因逆转录酶-聚合酶链反应用于克隆和确定构成该基因5 '端的另外306个核苷酸的序列。Aeact-1基因编码区核苷酸序列与D. melanogaster和B.家蚕肌动蛋白基因，其推导的氨基酸序列与这些基因的同源性在95%以上。Aeact-1基因在氨基酸序列水平上与B的相似性最高。mori和D.黑腹肌肌动蛋白Southern杂交分析表明，伊蚊基因组至少含有5个肌动蛋白相关序列。
A recombinant phagemid containing a 1,240-bp insert encoding an actin was isolated from a yellow fever mosquito, Aedes aegypti (L.), complementary DNA library. This insert (pBS-Act35) contained an open reading frame of 822 bp whose deduced amino acid sequence exhibited > 95% homology with the carboxyl terminal 274 amino acids of Drosophila melanogaster Meigen and silkworm, Bombyx mori (L.), actin genes. Reverse transcriptase-polymerase chain reaction was used to clone and determine the sequence of the additional 306 nucleotides that comprise the 5' end of the gene. The coding nucleotide sequence of the whole gene (designated Aeact-1) exhibited between 81 and 89% homology with coding sequences of D. melanogaster and B. mori actin genes, and its deduced amino acid sequence exhibited > 95% homology with those genes. The highest similarity of Aeact-1 gene at the amino acid sequence level was with B. mori and D. melanogaster muscle actins. Southern blot analysis indicated that the Aedes genome contains at least 5 actin-related sequences.